γ-辐射对T淋巴细胞环核苷酸昼夜节律的毒效应

Toxic Effects of γ-Irradiation on Circadian Rhythms of Cyclic AMP and Cyclic GMP in Mouse Blood Lymphocytes

测定了小鼠外周血Ｔ 淋巴细胞中ｃＡＭＰ和ｃＧＭＰ含量及其比值的昼夜节律， 各峰值时相分别位于１２∶２０ 时、０１∶１２ 时和１３∶０４ 时， 经５－０ Ｇｙ γ－ 射线照射后， ｃＡＭＰ含量升高，ｃＧＭＰ含量降低，ｃＡＭＰ／ｃＧＭＰ比值增大。而０－５Ｇｙ 照射引起比值下降。这些变化在０４∶００ 时较为明显， 而在１２∶００ 时则观察不到， 表现出明显的昼夜时相差异。另一方面， 照射后ｃＡＭＰ／ｃＧＭＰ比值节律的峰值位相发生延迟或提前。实验结果提示， 电离辐射对淋巴细胞ｃＡＭＰ和ｃＧＭＰ昼夜节律的影响，

Circadian rhythms of cyclic AMP,cyclic GMP and cAMP/cGMP were measured in mouse blood T lymphocytes.Upon 5Gy of γ-irradiation,the level of cAMP increased and that of cGMP decreased,leading to an augmentation of the cAMP/cGMP,while the low dose of 0\^5Gy caused a decline in the ratio.These changes,however,occurred only obviously at circadian time of 04∶00 and could not be observed at 12∶00.The acrophase of the cAMP/cGMP ratio was either delayed or advanced depending on the irradiation dosage.The experimental results indicated a mutual feature and a circadian characteristic of the irradiation effects on the cAMP/cGMP ratio in mouse blood T lymphocytes.