红花总黄素抑制血小板激活因子所致毛细血管通透性增加的研究

Study on the Inhibition of Safflor Yellow Against the Increase of Mouse Capillary Permeability Induced by Platelet Activating Factor

本文研究了红花总黄素（ＳＹ） 拮抗血小板激活因子（ＰＡＦ） 诱导的小鼠毛细血管通透性增加的作用。小鼠随机分为正常动物空白对照组（Ａ），模型动物对照组（Ｂ） ，模型动物大剂量（Ｃ） ，中剂量（Ｄ） ，低剂量（Ｅ） 药物组共五组（ｎ ＝ １２） 。Ａ，Ｂ组腹注ＮＳ，Ｃ，Ｄ，Ｅ组腹腔注射ＳＹ；３０ｍｉｎ 后，各组尾静脉注射伊文斯蓝；尾注后３０ｍｉｎ，Ａ组腿部肌肉注射ＮＳ，Ｂ，Ｃ，Ｄ，Ｅ组肌注ＰＡＦ造模；肌注３０ｍｉｎ 后处死小鼠。提取腿部肌肉中的染料，测定提取液６２０ｎｍ 的吸收度反映毛细血管通透性。比色结果：Ａ 组０－０８０ ±０－０４５ ，Ｂ组０－４５６ ±０－１５６（ 与Ａ组比较，Ｐ＜ ０－０１） ，Ｃ 组（１００ｍｇ／ｋｇ ＳＹ）０－２２２ ±０－０７１ ，Ｄ 组（７０ｍｇ／ｋｇ ＳＹ）０－３２２ ±０－１３５ ，Ｅ 组（５０ｍｇ／ｋｇ ＳＹ）０－３４２ ±０－１２９ ，（Ｃ，Ｄ，Ｅ，组与Ｂ 组比较依次为，Ｐ＜ ０－０１ ，０－０５ ，０－０５） ，提示ＳＹ 具有明显抑制ＰＡＦ诱发的小鼠毛细血管通透性增加的作用，并且这种作用随着ＳＹ

The inhibition of Safflor Yellow (SY)on the increase of the capillary permeability induced by platelet activating factor (PAF) in mice was investigated with Evans blue(EB) permeating colorimetry.The mice were divided randomly(n=12) into five groups:normal control group(A),PAF damaged group(B),SY high dose group(C),SY middle dose group(D)and SY low dose group(E).SY was injected intraperitoneally in group C,D,E and NS was injected the same way in group A,B; 30min later,in each group,EB was injected intravenously and 30min later PAF was injected into the leg muscle to make the PAF damaged model(group B,C,D,E),NS was given intramuscularly to the ones in group A; 30min after intramuscular injection,the mice were killed and the EB was extraced from the muscle with fomamide,then the absorbance at 620nm was determined.The results were showed as follows:group A:0.080±0.045,group B: 0.456±0.156(compared with group A, P <0.01),group C(100mg/kg SY):0 222±0.071,group D(70mg/kg SY):0.322±0.135,group E(50mg/kg SY):0.342±0.129.(group C,D,E compared with group B individually, P <0.01,0.05,0.05).It was by showed these data that the inhibition effect of SY against mouse capillary permeability increase induced by platelet activating factor(PAF) was dosage dependent.