慢性肺心病患者MDA、SOD和外周血淋巴细胞线粒体结构的观察

Study of MDA/SODand Structure Changes of Mifochondrion Membrane of Lymphocytein Patients with Cor Pulmonale

采用放射免疫法及硫代巴比妥酸荧光法分别检测了２０ 例慢性肺心病患者，１６ 例健康人的过氧化物歧化酶（ＳＯＤ） 、丙二醛（ＭＤＡ） ，并同时采用改良Ｂｏｙｕｎｎ 法分离固定淋巴细胞，对其淋巴细胞线粒体膜结构进行了研究。结果：肺心病病人的严重程度与ＳＯＤ含量、ＭＤＡ 水平、淋巴细胞线粒体数目及膜结构改变有相关性，心衰越重，ＭＤＡ 越高，淋巴细胞线粒体内的数目越多，线粒体嵴膜呈现不同程度的改变。提示：氧化与抗氧化失衡与肺心病患者淋巴细胞线粒膜结构的改变有一定的关系，可能是其影响细胞功能的靶部位，故对肺心病患者加强抗氧化的治疗，保持氧化与抗氧化的平衡状态。

The SOD, MDAand the structuresof mitochondrion membrane oflymphocytes were examinedin 20 patients with chroniccorpulmonale and 16 healthy controls.Theresultsshowed both thelevelsof SODand MDAand the numbers of mitochondrioninlymphocytes were significantly associated with the stages of cor pulmonale. The pa tients withlongerhistory and moreseverecorpulmonale had higherlevelsofSODand MDAand more numbersof mito chondriain theirlymphocytes. Theresultssuggested that MDA/SODimbalance correlated with the membrane struc tures of mitochondrioninlymphocytes.The mitochondrion membrane mightbethe oneofthetargetsofthisimbalance. Sothe patients with cor pulmonale might getsome benefitsfrom anti- oxidation treatment. Key words: Cor pulmonale;Lymphocyte; MDA;SOD; MitochondriaStudy of MDA/SODand Structure Changes of Mifochondrion Membrane of Lymphocytein Patients with Cor Pulmonale Yang Lan , Leng Lijia, Yu Xiulan, etal. Departmentof Respiratory, First Affitiated Hospitalof Xian MedicalUniversity, Xian (710061) Abstract The SOD, MDAand the structuresof mitochondrion membrane oflymphocytes were examinedin 20 patients with chroniccorpulmonale and 16 healthy controls.Theresultsshowed both thelevelsof SODand MDAand the numbers of mitochondrioninlymphocytes were significantly associated with the stages of cor pulmonale. The pa tients withlongerhistory and moreseverecorpulmonale had higherlevelsofSODand MDAand more numbersof mito chondriain theirlymphocytes. Theresultssuggested that MDA/SODimbalance correlated with the membrane struc tures of mitochondrioninlymphocytes.The mitochondrion membrane mightbethe oneofthetargetsofthisimbalance. Sothe patients with cor pulmonale might getsome benefitsfrom anti- oxidation treatment.