阻断缝隙连接增强丙泊酚和七氟烷的全身麻醉作用

Gap Junction Blockage Enhances the General Anesthesia Effects of Propofol and Sevoflurane

目的:观察缝隙连接阻断剂甘珀酸对丙泊酚和七氟烷全身麻醉作用的影响。方法:雄性Wistar大鼠80只随机分为8组,每组10只;空白对照组(C组)侧脑室注射生理盐水2μL后,腹腔注射生理盐水2mL;甘珀酸组(CBX组)侧脑室注射甘珀酸200μg后,腹腔注射生理盐水2mL;丙泊酚组(P组)侧脑室注射生理盐水2μL后,腹腔注射丙泊酚(5mg/100g);小剂量甘珀酸+丙泊酚组(CBX+P1组)侧脑室注射甘珀酸200μg后,腹腔注射丙泊酚(5mg/100g);中剂量甘珀酸+丙泊酚组(CBX+P2组)侧脑室注射甘珀酸300μg后,腹腔注射丙泊酚(5mg/100g);大剂量甘珀酸+丙泊酚组(CBX+P3组)侧脑室注射甘珀酸400μg后,腹腔注射丙泊酚(5mg/100g);七氟烷组(S组)予浓度梯度七氟烷;甘珀酸+七氟烷组(CBX+S组)侧脑室注射甘珀酸200μg后,给予浓度梯度七氟烷。观察并比较各组入睡时间、睡眠持续时间及入睡七氟烷浓度。结果:CBX+P1组、CBX+P2组及CBX+P3组入睡时间明显短于P组(P<0.01),睡眠持续时间明显长于P组(P<0.01);CBX+P2组及CBX+P3组入睡时间短于CBX+P1组(P<0.05);C组与CBX组大鼠均无睡眠征象;CBX+S组的入睡七氟烷浓度小于S组(P<0.05),但入睡时间和睡眠持续时间2组间差异无统计学差异。结论:缝隙连接阻断剂甘珀酸可增强丙泊酚和七氟烷的全身麻醉作用。

Objective： To assess the effects of carbenoxolone （CBX）, a gap junction bloc- ker, on the general anesthesia effects of propofol and sevoflurane. Methods： Eighty male Wistar rats were randomly divided into 8 groups （n= 10 each）. Rats in the control group （C group） re- ceived intracerebroventricular injection of normal saline solution （NS） 2 ttL and lumbar injection of NS 2 mL; rats in the CBX group （CBX group） received intracerebroventricular injection of CBX 200 ttg and lumbar injection of NS 2 mL; rats in the propofol group （P group） received in- tracerebroventricular injection of NS 2 ttL and lumbar injection of propofol （5 mg/100g） ; rats in the small dose CBX＋propofol group （CBX＋P1 group） received intracerebroventricular injection of CBX 200 ttg and lumbar injection of propofol （5 mg/100g）; rats in the middle dose CBX＋ propofol group （CBX＋P2 group） received intracerebroventricular injection of CBX 300 ttg and lumbar injection of propofol （5 mg/100g） ; rats in the high dose CBX＋propofol group （CBX＋P3 group） received intracereblovei^tdcular injection of CBX 400 ttg and lumbar injection of propofol （5 mg/100g） ; rats in the sevofl,rane group （S group） received gaseous anesthesia using sevoflu- rane with concentration gradient; rats in the CBX＋sevoflurane group （CBX＋S group） receivedintracerebroventricular injection of CBX 200 /~g and gaseous anesthesia using sevoflurane with concentration gradient. The latent period of falling asleep, the sustained period of sleep and the concentration of sevoflurane for sleep of different groups were compared. Results： The latent pe- riod of falling asleep of group （CBXq-P1）, （CBXq-P2） and （CBX^P3） were significantly shor- ter and the sustained period of sleep were significantly longer than those of group P （P^0.01）. The latent period of falling asleep of group （CBXq-P2） and （CBXq-P3） were shorter than that of group （CBXq-P1） （P%0.05）. No s!eep signs were detected in group C and CBX. The concentra- tion of sevoflurane for sleep in group （CBXq-S） was lower than that of group S （P%0.05）, but the latent period of falling asleep and the sustained period of sleep were not significant between two groups. Conclusion. CBX increase the general anesthesia effects of propofol and sevoflurane.