强直性脊柱炎患者血清OPG和sRANKL表达及意义

Expression and significance of serum OPG and sRANKL in patients with ankylosing spondylitis

目的探讨强直性脊柱炎(AS)患者血清护骨素(OPG)和可溶性核因子κB受体活化因子配体(sRANKL)表达及意义。方法采用ELISA检测44例AS患者与44例正常对照组血清OPG和sRANKL的水平,分析其与AS的相关性。结果血清sRANKL水平及sRANKL/OPG比值在AS组均显著高于正常对照组(P<0.01)。有外周关节受累的AS患者血清OPG水平显著高于无外周关节受累的AS患者(P<0.01)。最佳截断点上血清sRANKL(>5.24pmol/L)诊断AS的敏感性、特异性和正确率分别为54.5%、84.1%、69.3%,而sRANKL/OPG比值(>0.63)均为75.0%。结论 OPG/RANKL系统可能参与AS的发病和外周关节受累;血清sRANKL/OPG比值可以用于AS的辅助诊断。

Objective To investigate the expression and significance of serum osteoprotegerin（OPG）and soluble receptor activator of nuclear factor-кB ligand（sRANKL） in patients with ankylosing spondylitis（AS）.Methods Serum OPG and sRANKL levels were determined by ELISA in 44 AS patients（group A） and matched 44 healthy controls（group C）.Results Compared with group C,serum sRANKL and ratio of sRANKL to OPG were higher in group A than those in group C（P0.01）,which were higher in the cases with peripheral joint involvement than those without（P0.01）.According to Youden index of ROC curve,the diagnostic sensitivity,specificity and accuracy of cut-off point of serum sRANKL（5.24 pmol/L） were 54.5%,84.1%,and 69.3%,respectively,which of sRANKL/OPG ratio（0.63） were all 75.0%.Conclusion OPG/RANKL system may be involved in the pathogenesis of AS and peripheral joint injury.Serum sRANKL/OPG ratio may be taken as an auxiliary diagnosis indicator of AS.