白细胞介素-18mRNA在非小细胞肺癌中的表达及其临床意义

Expression of IL-18mRNA in non-small cell lung cancer and its clinical significance

目的:检测IL-18mRNA在非小细胞肺癌及癌组织中的表达,探讨其对非小细胞肺癌发生和发展过程的影响。方法:为非小细胞肺癌患者35例,男24例,女11例,年龄42-76岁,平均56岁,其中鳞癌17例(48.57%),腺癌13例(37.14%),腺鳞癌5例(14.29%)。对照组均来自距离肿瘤边缘2-4cm的正常肺组织。肿瘤直径≥4cm者6例(17.14%);肿瘤直径<4cm者29例(82.86%)。用RT-PCR法检测35例肺癌肿瘤组织细胞内和癌旁正常组织中的IL-18mRNA水平的表达。结果:在35例非小细胞肺癌中IL-18mRNA阳性表达率为100%,与癌旁正常组织比较,肺癌IL-18阳性表达率明显增高,差异有统计学意义(P<0.05)。结论:IL-18mRNA在NSCLC发展过程中发挥重要作用,其表达与患者年龄、性别和肿瘤组织类型无明显相关性,而与病理学分级、肿瘤T分期和有无淋巴结转移相关。

Objective：To study the expression of IL-18 expression in non-small cell lung cancer and the relationship with of the occurrence and development of non-small cell lung cancer.Medthods： Of 35 patients with non-small cell lung cancer there were 24 males and 11 females,aged from 42 to 76 years,mean 56,including 17 cases of squamous carcinoma（48.57%）,adenocarcinoma in 13 cases（37.14%）,adenosquamous carcinoma in 6 cases（14.29%）.The control group were normal lung tissue from the tumor margin 2-4cm,tumor diameter ≥ 4cm in 6 cases（17.14%）,4cm in 29 cases（82.86%）.IL-18 mRNA expression was detected by RT-PCR.Results： IL-18mRNA was positive in 35 cases of non-small cell lung cancer（100%）,compared with normal tissue,IL-18 mRNA positive expression was significantly increased,the difference was statistically significant（P〈0.05）.Conclusion： IL-18mRNA expression is no correlated with age,gender tumor type,but related with the pathological grade,tumor T stage and lymph node metastasis,IL-18 mRNA in NSCLC plays an important role in the development process.