RT-PCR法检测尿脱落细胞中XIAP的表达在膀胱癌诊断中的价值

Detection of XIAP in exfoliated urinary cells for diagnosis of bladder transitional cell carcinoma

目的:比较膀胱癌患者尿液脱落细胞中XIAP表达的RT-PCR检测法和常规尿脱落细胞病理学检测在膀胱癌诊断中的临床价值。方法:采用逆转录聚合酶链反应技术(RT-PCR)检测51例膀胱尿路上皮癌患者尿液脱落细胞中XIAP-mRNA的表达,同时行常规尿脱落细胞病理学检测,20例非肿瘤人员作为对照组。结果:实验组51例尿脱落细胞XIAP-mRNA RT-PCR检测阳性27例(53%),尿脱落细胞学病理学检测阳性12例(24%),对照组20例尿脱落细胞XIAP-mRNA检测阳性1例(5.0%),对照组尿脱落细胞病理学检测阳性0例(0%)。实验组RT-PCR检测膀胱尿路上皮癌患者尿脱落细胞中XIAP表达的敏感性高于尿脱落细胞病理学检测,差异有极显著统计学意义(P<0.01),实验组RT-PCR检测膀胱尿路上皮癌患者尿中XIAP表达的敏感性显著高于非肿瘤对照组,差异有极显著统计学意义(P<0.01)。结论:膀胱尿路上皮癌患者尿脱落细胞中XIAP表达的RT-PCR检测法较常规尿脱落细胞病理学检测更敏感,临床上作为膀胱癌的筛选方法,有一定的临床价值。

Objective：To compare the clinical value of XIAP detection in exfoliated urinary cells with exfoliated urine cytology in diagnosis of bladder transitional cell carcinoma.Methods： Expression of XIAP-mRNA in exfoliated urinary cells as well as exfoliative cytology were detected in 51 patients with bladder transitional cell carcinoma and 20 non-cancerous patients.Results： Of 51 patients 27（53%） with bladder transitional cell carcinoma showed positive XIAP in urine,while 12 of 51（24%）patients with bladder transitional cell carcinoma showed positive in exfoliated urine cytology.XIAP-mRNA expression detection in exfoliated urinary cells was significantly more sensitive than exfoliated urine cytology in patients of bladder transitional cell carcinoma（P〈0.01）,XIAP-mRNA expression detection in exfoliated urinary cells in patients of bladder transitional cell carcinoma was significantly more sensitive than those in non-cancerous patients（P〈0.01）.Conclusion： Detection of XIAP-mRNA is superior to exfoliated urine cytology in sensitivity and may be valuable in diagnosing bladder transitional cell carcinoma.