摘要
采用外周血液淋巴细胞培养法制备大额牛与云南黄牛杂交公牛的染色体标本,对大额牛与云南黄牛杂交公牛的染色体核型和G-带带型进行研究。结果表明,大额牛与云南黄牛杂交公牛染色体数目2n=59,其中2×28+1条常染色体中,t即2+28染色体为近中着丝粒染色体,其余2×27+1+1条均为端着丝粒染色体;1对性染色体中,X为近中着丝粒染色体,Y为一条小的端着丝粒染色体。大额牛与云南黄牛杂交公牛染色体数目2n=59,核型为:59,-2,-28,+t(2∶28),XY。
The chromosome samples and G-banding samples were made by peripheral blood lymphocyte culture to analyze the karyotype and G-banding pattern of the chromosomes in male hybrid of B.Frontalis×Yunnan Bos Taurus.Results showed that the number of the chromosomes in the male hybrid of B.Frontalis×Yunnan Bos Taurus was 59.There were 2×28+1 autosomes and 1 pair of sex-chromosome.Among 2×28+1 autosomes,t(2+28)was submetacentric chromosome,the others 2×27+1+1 autosomes were telocentric chromosomes.X chromosome was submetacentric chromosome,Y chromosome was a small telocentric chromosomes.The number of the chromosomes in the male hybrid of B.Frontalis×Yunnan Bos Taurus was 59,and the karyotype was 59,-2,-28,+t(2∶28),XY.
出处
《现代农业科技》
2011年第23期329-330,333,共3页
Modern Agricultural Science and Technology
基金
云南省自然科学基金(2008CD129)
关键词
大额牛
云南黄牛
杂交公牛
染色体
核型
G-带
B.Frontalis
Yunnan Bos Taurus
male Hybrid
chromosome
karyotype
G-banding