氮源对重组大肠杆菌发酵产L-精氨酸的影响

Effect of nitrogen sources on L-arginine production by recombinant E.coli

考察有机氮源种类、蛋白胨用量以及(NH4)2SO4用量对重组E.coli发酵产L-精氨酸的影响。结果表明:以蛋白胨作为有机氮源且用量在10 g/L,(NH4)2SO4用量在15 g/L时,摇瓶发酵产L-精氨酸产量最高,达到9.4 g/L。在5 L发酵罐进行补料分批培养,通过补加(NH4)2 SO4,L-精氨酸产量可以达到18.8 g/L,比未补加提高了108.9%。

The effect of nitrogen sources on L-arginine production by recombinant E.coli was studied.The effects of the varieties of organic nitrogen sources,peptone concentration,and（NH4）2SO4 concentration on L-arginine production were investigated.When the concentration of peptone and（NH4）2SO4 were 10 g/L and 15 g/L,respectively,9.4 g/L of L-arginine was obtained in shake flask culture.In batch fermentation with a 5-L fermentor,18.8 g/L of L-arginine was achieved by feeding（NH4）2SO4,thus it was 108.9% higher than that of control.