建立支气管哮喘大鼠模型的新方法及评价

Establishment and evaluation of animal model with bronchial asthma in rats

目的探讨建立支气管哮喘动物模型的新方法。方法 20只清洁级SD大鼠随机分为哮喘组和对照组,每组10只,哮喘组以小剂量卵蛋白(OVA)1mg致敏并激发为大鼠哮喘模型,对照组以氢氧化铝凝胶致敏,以0.9%氯化钠溶液激发。观察2组大鼠肺组织病理、支气管肺泡灌洗液(BALF)细胞分类及血清OVA-specific IgE水平。结果肺组织病理显示,哮喘组气道炎症明显;哮喘组BALF中中性粒细胞、淋巴细胞、嗜酸性粒细胞较对照组明显升高(P<0.05);哮喘组血清OVA-IgE水平较对照组显著升高,差异有统计学意义(P<0.05)。结论该方法成功的制备了支气管哮喘大鼠模型。

Objective To explore a new method to establish animal model with bronchial asthma in rats.Methods Twenty SD rats（SPF） were randomly divided into asthma group and control group,with 10 rats in each group.The rats in asthma group were sensitized by low-dose ovalbumin（OVA 1mg） to establish rat asthma models,however,the rats in control group were sensitized by aluminium hydroxide gel and were provoked by 0.9% sodium chloride solution.The pathological changes of lung tissue,the cell dividing in bronchoalveolar lavage fluid（BALF） and the levels of OVA-specific IgE in serum were detected and observed for both groups.Results The results of pathological examination showed that airway inflammation was severe in asthma group,and the ratios of neutrophil,lymphocyte and eosinophil in BALF in asthma group were significantly increased,as compared with those in control group（P〈0.05）,furthermore,the serum levels of OVA-specific IgE in asthma group was significantlt higher than that in control group（P〈0.05）.Conclusion The bronchial asthma models in SD rats are successfully established by the method in this investigation.