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IL-2Rα结合位点修饰型OviIL-2基因真核表达载体的构建 被引量：1

Cloning and Expression Vector Construction of a IL-2Rα Binding Related Sites Modified roviIL-2
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摘要 IL-2主要由激活后的T细胞产生,是一种天然的免疫增强剂,临床应用广泛。为了表达roviIL-2,本研究采用人工合成的方法获得基因,通过大肠杆菌对目的基因进行扩增筛选,并成功构建了适用于毕赤酵母表达系统的roviIL-2的真核表达载体pPIC9K-roIL2。本研究为下一步roviIL-2的真核表达及免疫机理研究奠定了基础。 IL-2 is mainly produced by activated T lymphocyte,act as a natural immune enhancer,and being widely used in clinic.In this study,a roviIL-2 gene was generated by artificial synthesis directly and transformed in E.coli C600.Based on this.We constructed a recombinant oviIL-2 expression vector pPIC9K-roIL2 apply to Pichia pastoris eukaryotic expression system.This studymade a solid foundation for further study of eukaryotic expression and immune mechanismof roviIL-2.
作者 王文昊 苗利光 李海涛 刘艳环 王松 安亚雄
机构地区 江苏科技大学 中国农业科学院特产研究所
出处 《特产研究》 2011年第4期5-8,共4页 Special Wild Economic Animal and Plant Research
基金 吉林省科技发展计划项目(20100219)
关键词 绵羊IL-2 基因克隆 真核表达载体 Ovine IL-2 Gene clone Eukaryotic expression vector
分类号 Q785 [生物学—分子生物学]
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参考文献10

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二级参考文献30

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特产研究
2011年 第4期

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