支气管哮喘患者呼出气冷凝液半胱氨酰白三烯C4及8异前列腺素水平与病情的相关性研究

The study between the level of LTC4,8-isoprostane in exhaled breath condensate and the serious condition of patients with asthma

目的探讨支气管哮喘患者呼出气冷凝液（EBC）中半胱氨酰白三烯C4（LTC4）及8异前列腺素水平与病情的关系。方法对哮喘患者观察组30例（哮喘慢性持续期患者16例，哮喘临床缓解期14例）用自行设计的EBC收集仪器采集标本，采用酶联免疫法测定EBC中LTC4及8异前列腺素水平。结果观察组中哮喘持续期及缓解期LTC4水平[（67．38±10．30）ng／ml、（41．21±11．41）ng／m1]均高于正常对照组[（17．15±7．48）ng/ml，P〈0．01]；哮喘持续期LTC4水平高于缓解期（P〈0．01）。哮喘持续期和缓解期8异前列腺素水平[（14．29±10．74）ng／ml、（12．40±7．45）ng／ml]显著高于正常对照组[（6．93±5．58）ng／ml]（P〈0．01或P〈0．05）；哮喘持续期8异前列腺素水平高于缓解期，其差异无统计学意义（P〉0．05）。结论哮喘熳性持续期和缓解期均有持续气道炎症存在，呼出气冷凝液（EBC）中LTC4水平与哮喘病情严重度有关，监测患者EBC中LTC4水平有助于哮喘的病情判断及指导治疗。

Objective To study the relationship between the level of LTC4, 8-Isoprostane in exhaled breath condensate and the serious condition of patients with asthma. Methods 30 patients with asthma were enrolled in this study, 16 cases were chronic persistent and 14 cases were clinical relieve. EBC was collected in all subjects with a self-designed experimental device. The concentrations of LTC4 and 8- Isoprostane in EBC were measured by enzyme immunoassay. Results Both LTCA [ （ 67.38 ± 10. 30） ng/ ml, （41.21 ± 11.41 ） ng/ml ] and 8-Isoprostane levels [ （ 14. 29 ± 10. 74） ng/ml, （ 12. 40 ± 7.45 ） ng/ml ] were significantly increased in the asthma patients compared with the control subjects[ （17. 15 ± 7.48 ）ng/ ml, P 〈 0. 01 ]. Exhaled LTC4 levels were obviously increased in the patients with chronic persistent asthma compared with those clinical relieve asthma, while 8-Isoprostane level had no significant difference between two asthma groups （ P 〉 0. 05 ）. Conclusions Airway inflammation were persistent in asthma patients. LTC4 and 8-Isoprostane levels in EBC were associated with the serious condition of asthma, which indicated inflammation indicators might be useful in monitoring serious condition and guiding treatment for asthma patients.