槐杞黄联合孟鲁司特钠干预对哮喘大鼠气道炎症以及IL-17表达的影响

Combined therapeutic effects of Huaiqihuang and Montelukast sodium on airway inflammation and expression of IL-17 in asthmatic rat

目的探讨槐杞黄辅助孟鲁司特钠治疗哮喘的作用机制。方法取40只雄性Spra—gue—Dawley（SD）大鼠随机分为对照组（Control）、模型组（Model）、孟鲁司特钠组（MK）、槐杞黄组（H）、孟鲁司特钠＋槐杞黄组（MK＋H），每组8只。除对照组外，其他各组建立哮喘模型。各药物干预组每天分别予以孟鲁司特钠、槐杞黄、孟鲁司特钠＋槐杞黄灌胃处理。取右肺上叶组织行病理学检查，分析气道炎症，免疫组化方法测定肺组织IL-17表达，计数BALF细胞总数和细胞分类，ELISA方法测定血浆及BALF上清液中IL-17的浓度。结果建模成功后（1）模型组支气管周围炎症细胞浸润程度较对照组显著增强（P〈0．05）；（2）各药物干预组支气管周围炎症细胞浸润程度较模型组明显减轻（P〈0．05）；（3）MK＋H组与MK组比较，支气管周围炎性细胞浸润程度明显减轻（P〈0．05），IL-17蛋白表达量（11．60±1．58）明显下降（P〈0．05），BALF细胞总数以及血浆和BALF中IL-17浓度均降低（P〈0．05）；（4）血浆与BALF中IL-17浓度及肺组织IL-17表达量呈正相关（P〈0．05）。结论槐杞黄颗粒联合孟鲁司特钠干预哮喘大鼠能进一步降低血浆及BALF中IL—17浓度，下凋肺组织IL—17蛋白的表达量，抑制气道炎症，其抗炎作用可能是通过下调IL-17来实现。

Objective To explore the mechanism of Montelukast combined with Huang Qi Huai in the treatment of asthma. Methods Forty male Sprague-Dawley （SD） rats were chosen and randomly divided into five groups： control group （Control） , model group （Model） , Montelukast group （MK） , Huang Qi Huai group （H） , Montelukast ＋ Huang Qi Huai group （MK ＋ H） , each group has 8 rats. The other four groups except the control group were built to chronic rat asthma model. The treatment groups were administered intragastrically with Montelukast, Huang Qi Huai and Montelukast ＋ Huang Qi Huai respectively. All animals were sacrificed; plasma and bronchoalveolar lavage fluid （BALF） and superior lobe of right lung tissues were collected. Superior lobes of right lung tissues were used to measure the expression of IL-17 in lung tissue by immunohistoehemistry. The airway inflammation was analyzed by histochemistry staining with H.E. Total cells score and differential score in BALF were counted. The levels of IL-17 in the plasma and BALF were measured by Enzyme-linked immunosorbent assay （ELISA）. Results Compared with the control group, the degree of inflammatory cell around the airway in the model group were significantly higher （ P〈 0.05 ）. Compared with group model, the degree of inflammatory cell around the airway in the Group MK, group H and group MK ＋ H were significantly ameliorated （ P 〈 0. 05 ）. Compared with Group MK, the degree of inflammatory cell around the airway in group MK ＋ H was significantly ameliorated （ P 〈 0. 05 ） , the expression of IL-17 in lung tissues was significantly lower （ P 〈 0. 05 ） , the numbers of total cells and the concentration of IL-17 in plasma and BALF were decreased too （ P 〈0. 05 ）. Correlation analysis showed that the level of IL-17 in the plasma and BALF was positively correlated with the level of IL-17 in the lung tissue（ P 〈 0. 05）. Conclusions Combined Huang Qi Huai adjuvant Montelukast treatment of asthmatic rat further reduced the concentration of IL-17 in the plasma and BALF, reduced the expression of IL-17 in lung tissue, improved the airway inflammation. Down regulation the expression of IL-17 was probably one of the mechanisms of anti-inflammatory.