摘要
目的:从分子水平探讨黄精属部分药用植物间的系统位置。方法:采用RAPD分子标记方法,以同科万寿竹属植物宝铎草(Disporum sessile D.Don)为外类群,对百合科黄精属19批(6种)药用植物进行基因组DNA的多态性分析,通过聚类分析探讨黄精种属间亲缘关系。结果:筛选出7条随机引物,聚类结果表明,黄精属植物在属级分类上特征明显,但属内分类有交叉。结论:RAPD技术可以为黄精属的分类和种的鉴别提供一定的理论依据。
Objective： To study the systematic position of some medicinal plants from genus Polygonatum through molecular technology. Methods： The DNA polymorphisms of 19 groups （6 species ） from genus Polygonatum and Disporum sessile D. Don as the outgroup were detected by RAPD molecular markers. Results： Seven arbitrary primers were screened for the RAPD analysis, phylogenetic trees revealed that the classified features of genus were obvious while the classification of inter - genus was crossed. Conclusion： RAPD markers may offer some evidence for the identification and categorization from genus Polygonatum.
出处
《中国野生植物资源》
2011年第6期34-37,共4页
Chinese Wild Plant Resources
关键词
黄精属
RAPD
聚类分析
系统位置
Polygonatum
RAPD
culster analysis
systematic position
