转化三七提取物为人参皂苷Rg_3的真菌分离鉴定

Isolation and identification of the fungi which can convert Panax Notoginseng extract into ginsenoside Rg_3

从培养人参(Panar ginseng C.A.Meyer)的土壤中筛选出23株菌,分别进行三七(PanaxNotoginseng)提取物转化实验.经过摇瓶发酵后,采用TLC法与HPLC法对其发酵液提取物进行分析.研究结果表明,能够转化三七提取物为高活性人参皂苷的微生物菌株有9株,其中以菌株TR-20的转化作用最强.进一步对其发酵产物分析表明,菌株TR-20能够转化三七提取物为人参皂苷Rg3,转化产物中人参皂苷Rg3含量为190.2 mg/L,RSD为0.69%(n=5).通过对菌株TR-20的形态和培养特征观察,表明菌株TR-20属于棒曲霉属(Aspergillus sp).

Twenty-three strains were isolated from soil where ginseng grew.The Panax Notoginseng extract were analyzed by TLC and HPLC.There were nine strains which can converte Panax Notoginseng extract into highly active ginsenoside,among which the TR-20 was the best one.Moreover,the TR-20 can convert Panax Notoginseng extract into ginsenoside Rg3（190.2 mg/L）.The TR-20 was Aspergillus（Aspergillus sp.） with observation of morphology and cultural characteristics.