黄花蒿内生菌的分离与初步鉴定

Isolation and Preliminary Identification of Endophytes from Artemisia Annua Linn.

利用平板分离法从药用植物黄花蒿(Artemisia annua Linn.)的根、茎和叶中共分离内生菌80株,其中内生真菌37株、细菌40株、放线菌3株。经菌种形态观察和染色等,初步鉴定了黄花蒿内生真菌具有5个属,包括囊孢菌(Capsule)、头孢霉(Cephalosporium)、弯孢霉(Curvularia)、曲霉(Aspergillus)和毛霉(Mucor),内生细菌有7个属,包括泛菌(Pantoea)、土壤杆菌(Agrobacterium)、假单胞菌(Pesudomonas)、芽孢杆菌(Bacillus)、巴斯德氏菌(Pasteurella)、葡萄球菌(Staphylococcus)、黄杆菌(Flavobacterium),内生放线菌有2个属,包括诺卡氏菌(Nocardia)和链霉菌(Streptomyces)。黄花蒿内生菌具有丰富多样性,但不同部位内生菌的数量、种类及分布存在差异。

Eighty strains were isolated from roots,stems and leaves of Artemisia annua using solidified plates,including 37 fungi,40 bacteria and 3 actinomycetes.The endophytic fungi belonged to 5 genera through morphological observation and staining,which were Capsule,Cephalosporium,Curvularia,Aspergillus and Mucor.The bacterial endophytes had 7 genera,including Pantoea,Agrobacterium,Pesudomonas,Bacillus,Pasteurella,Staphylococcus and Flavobacterium.The endophytic actinomycetes belonged to 2 genera,Nocardia and Streptomyces.Endophytes associated with Artemisia annua.had abundant biodiversity.However,different quantity and species of endophytes existed in roots,stems and leaves of A.annua Linn.