狗脊生、制品不同提取部位对成骨细胞的影响

Effect of Different Extracts From Cibotium barometz on Cultureing Osteoblasts

目的:探索狗脊不同提取部位对体外培养成骨细胞的增殖作用;比较狗脊生、制品对细胞增长的影响。方法:用狗脊生制品的石油醚层、氯仿层、乙酸乙酯层、正丁醇层及粗多糖与成骨细胞共同培养;并且将各提取部位给大鼠灌胃,取大鼠含药血清与成骨细胞共同培养,以MTT法检测细胞的增殖。结果:各提取部位与细胞共同培养试验和含药血清与细胞共同培养试验中,均以狗脊正丁醇提取部位对细胞增殖作用最明显,且制品强于生品。结论:狗脊炮制品正丁醇提取物能够显著促进成骨细胞增殖。

Objective：To explore the effects of different extracts from Cibotium barometz on proliferation of Osteoblasts.To compare the effects on proliferation of osteoblasts between crude and processed products,to explore the mechanism of anti-osteoporosis for C.barometz.Method：Osteoblasts with petroleum ether layer extract,chloroform layer extract,ethylacetate layer extract,butanol layer extract and polysaccharide from crude and processed products of C.barometz were cultured respectively.Meanwhile,after the rats were fed with each layer extract,the rat serum together with osteolastes was cultured.The proliferation was tested by MTT method.Result：In the different extract with osteoblasts test and the rat serum with osteoblasts test,the butanol layer extract had the most significant effect on cell proliferation of osteoblasts.And the effect of the processed product was more significant than that of crude product.Conclusion：The butanol layer extract from processed products of C.barometz had the most significant effect on cell proliferation of Osteoblasts.