不同制法四逆汤中甘草苷含量比较

Comparison of Liquiritin Content in Different Methods of Sini Decoction

目的:对四逆汤传统汤剂、经方颗粒剂及单味配方颗粒剂中甘草苷的含量进行比较。方法:采用高效液相色谱法,Diamonsil C18色谱柱(4.6 mm×150 mm,5μm),流动相乙腈-0.5%冰乙酸(20∶80),流速0.7 mL.min-1,检测波长276 nm,柱温25℃,测定3种剂型中甘草苷的含量。结果:甘草苷(每处方)在传统汤剂中含量为31.13 mg,经方颗粒剂中含量为48.48mg,单味配方颗粒剂中含量为31.95。结论:3种剂型中指标性成分甘草苷的含量有显著性差异。可为四逆汤剂型的进一步研究提供科学依据。

Objective：To compare the content of liquiritin in Sini traditional decoction,the granules and the ingle-flavor granules.Method：To determine the content of liquiritin by HPLC method Diamonsil C18 column（4.6 mm×150 mm,5 μm）was,used,the mobile phase of acetonitrile-0.1% acetic acid-water（20∶80）.The flonrate was 0.7 mL·min-1 and the detection wave length was at 276 nm.The tem perature of colcomn was 25 ℃.Result：The content of liquiritin was 31.13 mg in traditional decoction,48.48 mg in the granules and 31.95 mg in the ingle-flavor granules in a prescription.Conclusion：The content of liquiritin is significantly different formulations,which can provide the scientific basis for further research of Sini decoction type.