HPLC测定不同产地山药中腺苷含量

Determination of Adenosine in Dioscorea opposita from Different Cultivation Areas by HPLC

目的:分析不同产地的山药中腺苷的含量,为山药质量标准的建立提供参考。方法:采用高效液相色谱法,SunFire C18色谱柱(4.6 mm×250 mm,5μm);磷酸盐缓冲液(pH 6.5)-甲醇(85∶15)为流动相,流速1.0 mL.m in-1;检测波长260 nm。结果:10批不同产地山药中腺苷含量介于0.035 9～0.104 0 mg.g-1。结论:该方法操作简便,结果稳定可靠,可用于山药的质量评价。

Objective： To determine content of adenosine in Dioscorea opposita from the various cultivation areas.Method： A SunFire C18 Colum（4.6 mm×250 mm,5 μm） was used with Methanol-phosphate buffer（15∶85）as the mobile phase.The detective wavelength was set at 260 nm and the flow rate was 1.0 mL·min-1.Result： Ten batches of the samples were analyzed.The content of adenosine ranged from 0.035 9-0.104 0 mg·g-1.Conclusion： The method was simple,reproducible and reliable.It can be used to control the quality of D.opposita.