木香生品、麸煨品及活性单体对小鼠胃肠运动的影响

Effects of Aucklandiae Radix,Bran-roasted Products and the Two Active Monomer on Gastrointestinal Motility in Mice

目的:研究木香生品、麸煨品及2个活性单体对正常小鼠和脾虚小鼠胃肠运动的影响。方法:正常小鼠分为空白组、莫沙比利组、健胃消食片组、木香生品和麸煨品组(5,10,20 g.kg-1),空白组ig蒸馏水,其余各组ig相应药液,连续2周;利血平脾虚小鼠分为空白组、模型组、莫沙必利组、木香生品和麸煨品组(5,10,20 g.kg-1)、木香烃内酯组(1.25,2.5,5.0 mg.kg-1)和去氢木香内酯组(2.5,5.0,10.0 mg.kg-1),正常空白对照组sc 0.15 mg.kg-1生理盐水外,其他各组均sc 0.15 mg.kg-1利血平注射液,空白组和模型组同时ig生理盐水20 mL.kg-1,莫沙比利组,木香生品和麸煨品各剂量组ig药液20 mL.kg-1,木香烃内酯和去氢木香内酯各剂量组ip药液5 mL.kg-1,连续2周。测定小肠炭末推进率和炭末胃内残留率。结果:木香麸煨品(5,10 g.kg-1)、木香烃内酯和去氢木香内酯可明显促进脾虚小鼠胃排空,木香生品、木香烃内酯(5 mg.kg-1)对脾虚小鼠小肠推进有明显促进作用(P<0.05)。结论:木香生用理气作用强,治疗脾虚症状效果良好,其活性单体木香烃内酯发挥了较强作用。

Objective：To investigate the effects of Aucklandia lappa,bran-roasted products and the two active monomer on gastrointestinal motility in mice.Method： The normal mice were randomly divided into nine groups in average： blank control group,mosapride control group,Jianwei Xiaoshi pian control group,the raw and bran-roasted A.lappa group（5,10,20 g·kg^-1）.The blank control group was given distilled water and the other groups were given appropriate extracts by intragastic admistration once a day for two weeks.The mice with reserpine induced were divided into fifteen groups：blank control group,model control group,Mosapride control group,the raw and bran-roasted A.lappa group（5,10,20 g·kg^-1）,costunolide group（1.25,2.5,5.0 mg·kg^-1）and dehydrocostuslactone group（2.5,5.0,10.0 mg·kg^-1）.The blank control group were injected distilled water subcutaneously,the other groups were given reserpine by subcutaneous injection.The blank and model control groups were given distilled water.mosapride control group,the raw and bran-roasted A.lappa group（5,10,20 g·kg^-1）were given appropriate extracts by intragastic admistration（20 mL·kg^-1）,costunolide and dehydrocostuslactone groups were injected（5 mL·kg^-1） intraperitoneally for two weeks.Charcoal as a marker was orally administered to determine the propulsive function of small intestine and gastric emptying.Result： The results of gastric emptying and intestine propulsion experiments in normal mice suggested that the effects of the raw and bran-roasted A.lappa on gastric emptying is not significance.The raw materials（10,20 g·kg^-1） promoted the intestine propulsion（P0.05）.The bran-roasted products（5,10 g·kg^-1）,costunolide and dehydrocostuslactone had significance effects on gastric emptying for the reserpine treated mice,the raw materials and costunolide（5 mg·kg^-1）promoted the intestine propulsion for the reserpine treated mice（P0.05）.Conclusion： The raw A.lappa has effective in the promoting the circulation of qiand is benefit for spleen deficiency,the active conponent costunolide plays an important role especially.