黄芪注射液对兔脂肪来源的间充质干细胞体外增殖和细胞周期的影响

Effects of Astragalus Injection on Cell Cycle and in Vitro Proliferation of Rabbit Adipose-derived Mesenchymal Stem Cells

目的:观察黄芪注射液对兔脂肪来源的间充质干细胞(ASCs)体外增殖的作用。方法:提取家兔脂肪间充质干细胞,培养后,设实验组和对照组,实验组加入不同质量浓度黄芪注射液,对照组加常规培养液,用流式细胞仪观察细胞周期,用四唑盐(MTT)法测定细胞的增殖率,比较两组的细胞周期和细胞增殖率。结果:培养24 h后,对照组增殖期的细胞占(65.3±4.8)%,黄芪注射液组占(72.6±5.7)%,差异有统计学意义(P<0.05);培养48 h后,黄芪注射液0.390 6～200 g.L-1,细胞增殖(吸光度A)为(2.273±0.100)～(1.983±0.125),对照组为(1.341±0.424),表明实验范围内各浓度黄芪注射液对ASCs均有明显促进增殖的作用,两组比较差异有统计学意义(P<0.01)。结论:黄芪注射液有明显的促进兔ASCs体外增殖的作用。

Objective：To investigate the effects of astragalus injection on cell cycle and in vitro proliferation of rabbit adipose-derived mesenchymal stem cells（ASCs）.Method：ASCs were obtained from an experimental rabbit and cultured.Multi experimental and a control group were designed.Different concentrations of astragalus injection were added to the culture fluid in the experimental group,while a conventional culture method was employed in the control group.The distribution of the cell cycle was measured by flow cytometry and the proliferation rates of ASCs by MTT,and compared between the experimental and the control group.Result：After 24 hours of culture,the number of the cells during the proliferative phase was increased significantly from（65.3±4.8）% for the control group to（72.6±5.7）% for the experimental group,and the differences between each experimental and the control groups were not significant.After 48 hours,the optical density was（2.273±0.100）-（1.983±0.125） in each experimental group at 0.390 6-200 g·L-1 of astragalus,while（1.341±0.424） in the control group,and the differences between each experimental and the control groups were significant（P0.01）,suggesting astragalus at the concentrations within the experimental rang might have big promotion on proliferation of ASCs.Conclusion：Astragalus injection has significant promotion effect on the in vitro proliferation of rabbit ASCs.