天升元口服液对再生障碍性贫血模型小鼠血液T淋巴细胞异常和造血调控因子GM-CSF水平的影响

Effects of Tianshengyuan Oral Solution on Abnormality of T Lymphocyte Subsets and Level of GM-CSF, in Mice with Immune-Mediated Aplastic Anemia

目的:观察天升元口服液对免疫介导的再生障碍性贫血(immune-mediated aplastic anemia,IAA)模型小鼠外周血T淋巴细胞异常和正负造血调控因子水平的影响,以探讨其作用机制。方法:给予IAA模型小鼠天升元口服液(含生药6.2,12.4 g.kg-1.d-1)ig,14 d后,通过流式细胞术检测外周血CD4+,CD8+及其比值,并结合荧光免疫分析法检测外周血中造血正负调控因子(GM-CSF,TNF-α)的含量。结果:天升元口服液高剂量组CD8+及CD4+/CD8+分别为(15.89±8.32)%和(2.34±1.10),(n=7),模型对照组CD8+及CD4+/CD8+分别为(35.13±13.13)%和(0.92±0.33)(n=12),组间有非常显著差异(P<0.01～0.001);同时,天生元口服液高剂量组的GM-CSF含量(1 400±670)ng.L-1,(n=10),也显著高于模型对照组(665±276)ng.L-1,(n=12,P<0.01)。此外,TNF-α有一定程度的降低。结论:天升元口服液通过降低CD8+T淋巴细胞的产生,提高CD4+/CD8+比值,促进T淋巴细胞亚群的平衡,同时促进造血正调控因子(GM-CSF)的产生,从而呈现对IAA模型小鼠的治疗作用。

Objective： To explore the regulation of Tianshengyuan oral solution（TSY） for the abnormality of T lymphocyte subsets and the level of hematopoietic cellular factors in mice with immune-mediated aplastic anemia（IAA）.Method： T-cell subsets,including CD4＋,CD8＋,in peripheral plasma were analyzed by flow cytometry.GM-CSF and TNF-α were measured by fluorescence immunoassay.Result： TSY（12.4 g·kg^-1·d^-1） ig for 14 days could significantly reduce the production of peripheral CD8＋ [（15.89±8.32）%,n=7,P〈0.01],increase in the ratio of CD4＋/CD8＋（2.34±1.10,n=7,P〈0.001） and in the level of GM-CSF[（1 400±670） ng·L-1,n=10,P〈0.01],compared with IAA model group[（35.13±13.13）%,（0.92±0.33）,and（665±276） ng·L-1,n=12,respectively].Meanwhile,the decreased levels of TNF-α were also seen in the same group.Conclusion These results suggest that TSY for the treatment of IAA mice could be by modulating the balance between T-lymphocyte subsets,and the levels of hematopoietic cellular factors.