紫叶李叶片花色素及花色苷HPLC定量分析

Quantitative Analysis of Anthocyanidin and Anthocyanin from Fresh Leaves of Prunus cerasifera var.atropurpurea by HPLC

利用高效液相色谱(HPLC)法首次定量分析了紫叶李鲜叶片中芍药色素(Peonidin)、花青素3,5-二葡萄糖苷(Cyanidin 3,5-di-O-glucoside)、天竺葵色素3,5-二葡萄糖苷(Pelargonidin 3,5-di-O-glucoside)、芍药色素3-葡萄糖苷(Peonidin 3-O-glucoside)、飞燕草素(Delphinidin)、花青素(Cyanidin)和锦葵色素(Malvidin)的存在。花青素3-葡萄糖苷(Cyanidin 3-O-glucoside)、芍药色素和色素12是紫叶李鲜叶片中三种重要色素,其相对含量均超过检测到色素总含量的10%,分别占检测到色素总含量的27.54%、24.08%和14.95%,鲜叶片中实际含量分别为3.396、2.969、2.108 mg/g。除芍药色素3-葡萄糖苷极微量外,花青素3,5-二葡萄糖苷、锦葵色素、飞燕草素、天竺葵色素3,5-二葡萄糖苷和花青素在鲜叶片中的含量分别为0.005、0.0075、0.0185、0.3590、0.0360 mg/g。其它28种色素的结构和含量需要进一步分析。

The contents of Peonidin,Cyanidin 3,5-di-O-glucoside,Pelargonidin 3,5-di-O-glucoside,Peonidin 3-O-glucoside,Delphinidin,Cyanidin and Malvidin in fresh leaves of Prunus cerasifera var.atropurpurea were quantitatively analyzed by HPLC.Cyanidin 3-O-glucoside,Peonidin and No.12 pigment were the principal pigments with the relative contents of 27.54%,24.08% and 14.95% respectively,whose actual contents in fresh leaves were 3.396,2.969 and 2.108 mg/g respectively.The contents of Cyanidin 3,5-di-O-glucoside,Malvidin,Delphinidin,Pelargonidin 3,5-di-O-glucoside and Cyandin in fresh leaves were 0.005,0.0075,0.0185,0.359 and 0.036 mg/g respectively,except Peonidin 3-O-glucoside,whose content was very low.The structure and content of the other 28 kinds of pigments needed further analysis.