花生壳中木犀草素的超临界CO_2萃取工艺研究

Extraction technology of luteolin from peanut hull by supercritical CO_2

目的:研究超临界CO2萃取花生壳中木犀草素的最佳工艺条件。方法:采用HPLC法测定木犀草素含量,以提取率为指标,应用单因素和正交实验,分别考察萃取温度、萃取压力、分离温度、分离压力以及CO2流量等5个因素影响,并将筛选工艺与目前文献的乙醇提工艺进行对比分析。结果:超临界CO2萃取木犀草素的最佳工艺条件:萃取温度为50℃,萃取压力为35MPa,分离温度为30℃,分离压力为10MPa,CO2流量为7L/h。结论:与传统乙醇提取工艺相比,超临界CO2萃取工艺提取花生壳中木犀草素的提取率提高了2.63倍。

Objective：The optimum supercritical CO2 extraction of luteolin from peanut hull were determined.Methods：HPLC method was used to determine the luteolin,experiments with single factor and orthogonal design,5 factors were studied on Supercritical CO2 extraction：extraction temperature,extraction pressure,separation temperature,separation pressure,CO2 flow rate.Results：The optimum extraction extraction were extraction temperature 50 ℃,extraction pressure 35 MPa,separation temperature 30 ℃,separation pressure 10 MPa,CO2 flow rate 7 L/h.Conclusion：Compared with the traditional ethanol extraction,the extraction yield of luteolin 2.15 times.