利用ISSR标记对93份广东桑桑树种质资源的遗传多样性分析

Analysis of Genetic Diversity of 93 Mulberry Germplasm Resources of Morus atropurpurea Roxb. Using ISSR Markers

应用ISSR分子标记技术分析广东桑(Morus atropurpurea Roxb.)桑树种质资源的遗传多样性,为桑树种质资源保存、鉴定及新品种选育提供基础依据。以13个ISSR引物从93份广东桑桑树种质材料的基因组DNA中共扩增出128条带,其中多态性条带94条,多态性比率为73.44%,表明供试的广东桑桑树种质材料间存在丰富的遗传多样性。93份广东桑桑树种质材料间的遗传相似系数为0.585 9～0.937 5,平均为0.761 7。基于供试种质材料间的遗传相似系数,采用UPGMA法对93份广东桑桑树种质材料的遗传关系进行聚类分析,93份种质材料在遗传相似系数0.664处被划分为6个组群,在遗传相似系数0.685处,第Ⅲ、Ⅳ、Ⅵ组群分别被分成2个亚组。研究结果显示,供试广东桑桑树种质材料的亲缘关系与地理分布、花性等存在关联。

In this study,the genetic diversity of mulberry germplasm resources of Morus atropurpurea Roxb.was investigated by means of ISSR molecular marking technology,which will provide fundamental references for the conservation and identification of mulberry germplasm resources,and the breeding of new varieties.Totally 128 bands were amplified from genomic DNA of 93 germplasm materials of Morus atropurpurea Roxb.using 13 ISSR primers and there were 94 polymorphic bands with a percentage of polymorphic band of 73.44%,implying that there was rich genetic diversity among the tested germplasm materials of Morus atropurpurea Roxb..Genetic similarity coefficients between the 93 germplasm materials of Morus atropurpurea Roxb.ranged from 0.585 9 to 0.937 5,with an average of 0.761 7.The genetic relationship between these germplasm materials was analyzed with UPGMA method based on their genetic similarity coefficients.The result showed that the 93 germplasm materials could be clustered into 6 groups at genetic similarity coefficient of 0.664,in which groups Ⅲ,Ⅳ and Ⅵ could be further divided into 2 subgroups respectively at genetic similarity coefficient of 0.685.The obtained results demonstrated that the genetic relationship of the tested germplasm materials of Morus atropurpurea Roxb.is correlated to geographical distribution and sex of flower of each germplasm material.