甲醛分子印迹聚合物微球的制备及其在针式萃取-气相色谱联用技术中的应用

Preparation of Formaldehyde Molecularly Imprinted Polymer Beads and Its Application in Needle Trap Extraction Coupled to Gas Chromatography

本研究选用甲醛为印迹分子,4-乙烯基吡啶为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用矿物油包被悬浮聚合法制备高选择性识别的分子印迹聚合物。与本体聚合法相比,此法很好地保护了聚合物的印迹位点,得到的微球形状规则。利用制备的聚合物微球作为吸附剂填充针式萃取装置,并与气相色谱联用分析了气体中的痕量甲醛。实验考察了一系列聚合反应条件参数对聚合物微球吸附能力的影响。结果表明:模板分子、功能单体和交联剂的物质的量之比为1∶4∶20,预聚合时间为3h,聚合溶液与矿物油配比为1∶6.5,聚合反应时间为8h时,制备的分子印迹聚合物对甲醛有良好的萃取性能。

In this study,the suspension polymerization in mineral oil method using formaldehyde as the template molecule,4-vinyl pyridine（4-VP） as the functional monomer and ethylene dimethacrylate（EDMA） as the cross-linker for the preparation of high selective molecularly imprinted polymer（MIP） beads was described.The prepared beads were spherical and regular with effective recognition sites compared to irregular shaped particles prepared by bulk polymerization.A needle trap extraction（NTE） device was prepared using the MIP beads as adsorption medium,and the device could be coupled with gas chromatography（GC） for the analysis of trace formaldehyde.A series of parameters concerning the effect of polymerization conditions on adsorption property have been evaluated.The results demonstrated that the best adsorption performance of the MIP beads was achieved under the optimized conditions with the reagents ratio（template molecule∶functional monomer∶cross-linker） of 1∶4∶20,pre-polymerization time of 3 h,the pre-polymerization solution to mineral oil ratio of 1∶6.5,and polymerization time of 8 h.The method using a combination of molecular imprinting and NTE technique would possibly provide a new way for the development of novel sample preparation techniques.