共振光散射法测定对乙酰氨基酚

Determination of Paracetamol by Resonance Light Scattering Technique

在酸性介质中,对乙酰氨基酚将铁氰酸根离子还原成亚铁氰酸根离子,后者与硫酸锌反应生成K2Zn3[Fe(CN)6]2粒子引起体系的共振光散射信号显著增强,且波长345nm处增强的散射信号强度△IRLS与对乙酰氨基酚的浓度在0.01～1.0!g/mL范围内呈良好线性关系。其线性回归方程为△IRLS=-15.01+4214c(c,!g/mL),相关系数(r)为0.9992。该方法具有较高的灵敏度,检出限(3σ)为7.4ng/mL。用于对乙酰氨基酚片剂的测定,相对标准偏差≤2.8%。

In acidic medium,ferricyanide ion was reduced to ferrocyanide ion by paracetamol（PCT）.The thus-gererated ferrocyanide ion reacted with Zn2＋ to form K2Zn3[Fe（CN）6]2 particles,which enhanced the resonance light scattering（RLS） intensity greatly.The enhanced RLS intensity at 345 nm is proportional to the content of paracetamol in a range of 0.01～1.0 μg/mL.The linear regression equation is △IRLS=-15.01＋4214c（c,μg/mL）.The correlation coefficients r is 0.9992.The limit of detection is 7.4 ng/mL.Based on these results,a novel resonance light scattering method for paracetamol was established.This method has been applied successfully to the determination of paracetamol in tablets with the RSD ≤2.8%.