氯化锂-紫外-离子束复合诱变红霉素高产菌株研究

Screening of Erythromycin High-Yielding Strain by Compound Mutation of LiCl,UV and Ion beam

以红霉素生产菌红色链霉菌为研究对象,探讨了氯化锂、紫外、离子束3种不同诱变方式对红色链霉菌的影响,根据后代的存活率和突变率选定合适的诱变条件:氯化锂的最优诱变浓度1.2%,紫外的最优诱变时间120s,离子束的最优诱变注量200×1014 cm-2,然后将3种方式结合起来进行复合诱变。经复合诱变后筛选获得高产突变株E0317-1-7-26,该菌株摇瓶效价达7 126μg/mL,较出发菌株E03提高了20.3%;经多次传代分离实验表明,该突变株遗传稳定性较好。

Taking erythromycin producing strain,Streptomyces erythreus as research object,the effects on Streptomyces erythreus caused by three different mutantion ways of LiCl,UV and ion beam were explored.The mutated conditions were optimized by survival rate and mutation rate.The optimum mutation concentration of LiCl is 1.2%,the proper mutation time of UV is 120 s,and the optimal fluence of ion beam is 200×1014 cm-2.Then the three ways were combined to do compound mutation.One high-yield mutant E0317-1-7-26 by compound mutation was obtained.The titer of the strain is 7 126 μg/mL,which is nearly 20.3% higher than that of original strain E03.Several passages and separation experiments show that the genetic stability of the mutants is good enough.