补阳还五汤指纹图谱总量统计矩加合性的研究

Study of additive properties of total quantum statistical moment of chromatographic fingerprint for Buyang Huanwu decoction

目的:采用总量统计矩法分析不同溶剂处理所获得的补阳还五汤HPLC指纹图谱,验证总量统计矩法的加合性。方法:采用水提醇沉法获取补阳还五汤总浸出物,依次用溶度参数11.4～23.40 Cal1/2.cm-3/2的5种溶剂溶解制样;测定各溶剂浸出物的HPLC指纹图谱,计算并分析其总量统计矩参数的叠加规律。结果:以补阳还五汤总方液指纹图谱为参照峰,用正丁醇(11.4 Cal1/2.cm-3/2)、甲醇(13.5 Cal1/2.cm-3/2)、68%甲醇(16.67 Cal1/2.cm-3/2)、34%甲醇(20.03 Cal1/2.cm-3/2)、水(23.40 Cal1/2.cm-3/2)依次溶解所获得的浸出物指纹图谱的相似度分别为0.074,0.973,0.934,0.991,0.993,其总量零阶矩、一阶矩、二阶矩的RSD为63.04%,16.22%,69.38%,表明各指纹图谱差异性较大;按指纹图谱加合性公式计算的总量零阶矩、一阶矩、二阶矩分别为3.203×105 mAu.s,29.85 min,389.97 min2,而实际测得的总方浸出物指纹图谱的总量零阶矩、一阶矩、二阶矩分别为6.548×104 mAu.s,29.44 min,389.00 min2;两者极差绝对值百分率分别为2.209%,1.389%,0.248 4%。结论:中药指纹图谱总量统计矩法具有加合性,可用于中药多成分体系静态与动态的质量监控分析。

Objective： To study the differences of HPLC fingerprints with the total quantum statistical moment for the Buyang Huanwu decoction （BYHWD） processed by various ways, and to verify the additive properties of total quantum statistical moment. Method： The extracts of BYHWD were obtained by water extraction and alcohol precipitation firstly, and then were dissolved with five solvents of different solubility parameter from 11.4 Cal^1/2·cm^-3/2to 23.40 Cal^1/2·cm^-3/2respectively. Their chromatographic fingerprints were determined by HPLC, finally the total quantum statistical moment parameters and its superposition properties were manual calculated and analyzed by their expressions. Result： As a contrast as the whole prescription, the similarities of the various processed samples with butanol（11.4 Cal^1/2·cm^-3/2）, methanol（13.5 Cal^1/2·cm^-3/2）, 68% methanol （16.67 Cal^1/2·cm^-3/2）, 34% methanol（20.03 Cal^1/2·cm^-3/2） and distilled water（23.40 Cal^1/2·cm^-3/2） were 0.074, 0.973, 0.934, 0.991, 0.993, respectively and while the RSD of these total quantum zero moment, center moment and variance of each chromatographic fingerprints for them were 63.04%, 16.22%, 69.38%, which showed significant difference in these chromatographic fingerprints. The total quantum statistical moment parameters of the superimposed chromatographic fingerprint with each sole samples were 3.203×105 mAu·s, 29.85 min, 389.97 min^2, whereas the whole prescription’s were 6.548×104 mAu·s, 29.44 min, 389.00 min^2, that suggested that the absolute difference percentages between the superimposed and the whole were 2.209%, 1.389%, 0.2484%, respectively. Conclusion： The total quantum statistical moment of the chromatographic fingerprints is of characteristics with additive properties, it can be used in static and dynamic quality controlled analyses in the Chinese medicine multiple component systems.