响应面法优化车前子油的提取工艺及抗氧化活性研究

Optimization of supercritical CO_2 extraction of Plantaginis Semen oil by response surface methodology and studies on its antioxidant activity

目的:优化超临界CO2萃取车前子油的最佳工艺条件,研究车前子油中脂肪酸的组成成分和含量以及其抗氧化活性。方法:在单因素实验基础上,利用响应面法,以萃取率为响应值,建立数学模型,获得最佳工艺;利用GC-MS分析车前子油中脂肪酸组成及含量,并采用自由基清除能力体系初步评价车前子油的抗氧化活性。结果:通过二次回归模型响应面分析得出最佳超临界CO2萃取车前子油最佳工艺条件是萃取温度70℃,萃取压力30 MPa,萃取时间120 min,CO2流速30 L.h-1,在此最佳工艺条件下车前子油萃取率为35.07%,理论值为35.91%,与理论值相对误差小于5%。经GC-MS分析得车前子油主要成分为亚麻酸、亚油酸、硬脂酸和棕榈酸,其中不饱和脂肪酸含量超过88%。抗氧化活性研究中,由DPPH.+和ABTS.+自由基清除实验得IC50分别为1.13,3.57 g.L-1。结论:由响应面法优化得到的车前子的提取工艺方便可行,得到的车前子油具有较强的抗氧化活性,值得进一步开发应用。

Objective： The optimum conditions of SFE-CO2 extraction of Plantaginis Semen oil （SPO）, the composition of SPO and its antioxidant activities of SPO were all investigated in this paper. Method： Response surface method （RSM） was used to establish the mathematical model of SFE-CO2 extraction of SPO to obtain the optimum conditions based on Single factor experiments. Fatty acid compositions and contents of SPO were tested by GC-MS, and antioxidant activities of SPO were studied by DPPH and ABTS free radical elimination method. Result： The optimum conditions obtained through RSM analysis were as follows： extraction tempreture 70 ℃,extraction pressure 30 MPa, extraction time 120 min and flow rate 30 L·h^-1. Under the optimal condition, predicted value was 35.91%, while the experimental value was 35.07%. The experimental values agree with the predicted from the regression model with a relative error less than 5%.The main components of SPO were 9,12,15-octadecatrienoic acid, 8,11-octadecadienoic acid,octadecanoic acid and hexadecanoic acid.Most of the fatty acids were polyunsaturated fatty ones,whose quantities were obtained more than 88%. The IC50 DPPH and IC50 ABTS were 1.13, 3. 57 g·L^-1 respectively in DPPH and ABTS assay. Conclusion： Optimization of the extraction process by RSM of SPO is convenient and feasible.SPO has good antioxidant activity and is worth to develop for application.