丹参酮ⅡA对糖尿病大鼠主动脉内皮细胞非对称性二甲基精氨酸和一氧化氮的影响

The Effect of Tanshinone ⅡA on Asymmetry Dimethyl Arginine and Nitric Oxide in Aortic Endothelial Cells of Diabetic Rats

目的观察丹参酮ⅡA对糖尿病大鼠主动脉内皮细胞非对称性二甲基精氨酸和一氧化氮的影响。方法制备2型糖尿病大鼠模型,组织块法原代培养大鼠主动脉内皮细胞,丹参酮ⅡA 0、25、50 mg/L干预细胞6 h、12 h和24 h,采用ELISA检测细胞培养基非对称性二甲基精氨酸含量,硝酸还原酶法检测一氧化氮含量。结果丹参酮ⅡA抑制内皮细胞分泌非对称性二甲基精氨酸的作用呈剂量、时间依赖性(P<0.05)。随浓度增高,其促进一氧化氮分泌的作用增强,于12 h时促进作用达高峰(P<0.05)。结论丹参酮ⅡA能降低糖尿病大鼠主动脉内皮细胞分泌非对称性二甲基精氨酸,同时促进一氧化氮的合成。

Aim To observe the effect of tanshinone ⅡA on asymmetry dimethyl arginine and NO in aortic endothelial cells of diabetic rats. Methods The diabetic model rats was induced and its aortic endothelial cells were primary cultured by tissue patch and intervened with 0,25,50 mg/L Tanshinone ⅡA sulfonate for 6 h,12 h and 24 h.The contents of asymmetry dimethyl arginine and NO in cell culture were determined by ELISA method and nitrate reductase method,respectively. Results The inhibiting effect of Tanshinone ⅡA sulfonate on asymmetry dimethyl arginine was in a dose-and time-dependent manner.The difference between each group had statistical significance（P0.05）.The promoting effect of secretion of NO was parallel to the increase of concentration,and it reached the peak in 12 h（P0.05）.Conclusion Tanshinone ⅡA can decrease the secretion of asymmetry dimethyl arginine in aortic endothelial cells and promote the synthesis of NO.