人工microRNA抗乙型肝炎病毒效果初探

Artificial microRNA Can Suppress Hepatitis B Virus Replication Efficiently In Vitro

目的:设计靶向乙型肝炎病毒(HBV)基因保守区的人工microRNA(amiRNA),考察其对HBV基因表达的抑制作用。方法:比对HBV全基因组现有序列,选择保守区设计amiRNA,定向克隆到pcDNA6.2-GW/EmGFP-miR载体,将amiRNA载体与HBV复制载体pHBV1.31共转染HepG2细胞,72 h后收取细胞上清,ELISA检测HBV表面抗原(HBsAg)及e抗原(HBeAg)的含量,荧光定量PCR检测HBV DNA含量。结果:amiRNA可显著抑制细胞上清HBsAg、HBeAg和HBV DNA的水平。结论:amiRNA作为防治HBV感染的潜在有效手段之一值得进一步深入研究。

Objective： To screen artificial microRNA（amiRNA） which can inhibit hepatitis B virus（HBV） replication in vitro.Methods： amiRNA targeted HBV conserved regions were designed and cloned into the vector pcDNA6.2-GW/EmGFP-miR.The inhibition rate of different amiRNA were compared by cotransfection amiRNA vectors and the HBV replicon vector pHBV1.31 into HepG2 cells.After 72 h,HBsAg,HBeAg and HBV DNA were assayed and analyzed.Results： amiRNA can suppress HBV replication efficiently in vitro.Conclusion： amiRNA as a potential anti-HBV technology shound be studied deeply in future.