云南栽培种及野生种芋头的AFLP指纹分析

AFLP Finger Analysis Between Cultivars and Wild Type of Taros(Colocasia esculenta) from Yunnan,China

目的:分析云南芋头种质资源遗传多样性。方法:应用扩增片段长度多态性(AFLP)指纹技术,用3对AFLP引物对采集自云南省的9份芋头栽培品种及1份野生品种进行研究,分离AFLP多态性条带。结果:共分离到60个AFLP多态性条带,AFLP多态位点百分率为96.77%,云南芋头种质资源在DNA分子水平上表现出丰富的遗传多样性。聚类分析将10份芋头品种分为2组,遗传距离为0.101～0.908。结论:AFLP指纹技术是筛选品种间差异基因的有效方法,研究结果为云南省芋头品种鉴定、遗传相关性分析、特殊功能基因的分离等工作提供了一定的理论基础。

Objective： To identify genetic diversities of taro germplasms in Yunnan province,China.Methods： The amplified fragmeng length polymorphism（AFLP） fingerprinting technology was used to analyze nine cultivars and one wild taro sampled in Yunnan province and to screen AFLP polymorphic bands.Results： 60 AFLP polymorphic bands were screened through three pairs of AFLP primes among these taros varieties and the ratio of polymorphic loci was 96.77%.It showed rich genetic diversities in taro germplasms of Yunnan province.The UPGMA result showed that these taros can be divided into two main groups,genetic distance among the collected taros range from 0.101 to 0.908.Conclusion： AFLP fingerprinting is an effective method to isolate special genes within different taro varieties.In summary,our study provides the theoretical basis for identification of taro variety and their genetic relationship in Yunnan.