山东地区汉族人群杀伤细胞免疫球蛋白样受体基因型P、AE、AG和单体型1、6、17与梅毒的关系

Association between KIR genotype P,AE,AG and haplotype 1,6,17 and syphilis in the Han population in Shandong area,China

目的分析山东地区汉族梅毒患者杀伤细胞免疫球蛋白样受体(KIR)基因型和单体型的多态性,探讨其与梅毒发生之间的关联性。方法采用序列特异性引物聚合酶链反应(PCR-SSP)法,对山东汉族梅毒患者190名和健康对照者192名的KIR基因进行检测和分析。结果共检出KIR基因型25种,其中包括新的基因型11种。25种KIR基因型共由12种单体型和6种未知单体型组成。在健康对照组中,KIR基因型P的频率显著高于梅毒患者组(P<0.05),其OR值为0.304;在梅毒患者组中,基因型AE和AG的频率显著高于健康对照组(P<0.05)。在健康对照组中,单体型17的表型频率显著高于梅毒患者组(P<0.05),其OR值为0.321;在梅毒患者组中,单体型1和6的表型频率显著高于健康对照组(P<0.05)。单体型A和B在这2组人群的分布频率差异相比无统计学意义(P>0.05)。结论 KIR基因型AE和AG可能是梅毒的易感基因型,基因型P可能是梅毒的抗性基因型;KIR单体型1和6可能是梅毒的易感单体型,单体型17可能是梅毒的抗性单体型。

Objective To explore whether KIR genotype and haplotype polymorphisms are associated with syphilis in Chinese Han population of Shandong area. Methods Polymerase chain reaction with sequence-specific primers （ PCR- SSP） method was used to genotype KIR genes in 190 syphilis patients and 192 healthy controls. Results The frequency of genotype P was higher in healthy controls than that in syphilis patients （ P 〈 0.05 ） and the OR was 0.304 ; while the frequencies of AE and AG were higher in syphilis patients than those in healthy controls. The frequency of haplotype 17 was lower, whereas, the frequencies of haplotype 1 and 6 were higher in syphilis patients than those in healthy controls. Conclusion Genotypes AE and AG are syphilis susceptible genotypes;P, on the other hand, may be a protective genotype from syphilis. The haplotype 1 and 6 are syphilis susceptible haplotypes, whereas, haplotype 17 may be the protective haplotype from syphilis in the Chinese Han population in Shandong area.