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小麦转录因子TaDREB6基因启动子的克隆及活性分析 被引量：3

Cloning and Activity Analysis of TaDREB6 Promoter in Wheat
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摘要 启动子的分析有助于解析植物抵御不良环境的机制。植物DREB转录因子参与对干旱、低温和高盐等胁迫的响应,在抗逆中起着重要作用。本研究利用反向PCR方法从小麦中克隆获得DREB转录因子TaDREB6基因启动子,长度为1 705 bp。PLACE和PlantCARE分析发现,TaDREB6基因启动子包含多种胁迫相关元件。构建由TaDREB6基因启动子驱动的GUS植物表达载体,转化小麦成熟胚愈伤组织,组织化学染色结果表明,TaDREB6基因启动子是诱导型启动子,受干旱、低温、盐、脱落酸和水杨酸等胁迫诱导。Real-time PCR显示,TaDREB6基因受多种胁迫诱导表达,与TaDREB6启动子活性分析结果一致,这些结果为进一步分析DREB转录因子的功能提供了依据。 DREB（dehydration responsive element binding） transcription factors response to drought,low temperature,and high salt etc and help plants survive in environmental stresses.To analyze the molecular mechanism of DREB,TaDREB6 promoter was cloned by inverse PCR from wheat genome,with full-length of 1705 bp.Many basic cis-acting elements related to various environmental stresses and plant hormones were found in the promoter sequence by PLACE and PlantCARE.To analyze the promoter activity,plant transient expression vector fused with the GUS（β-glucuronidase） gene,driven by TaDREB6 promoter was constructed and transformed into mature embryo calli of wheat.The calli were treated by drought,ABA,high salinity,SA,and low temperature and gave transient expression at different degrees.The TaDREB6 expression levels increased when treated with drought,low temperature,ABA,SA and NaCl.These results indicated TaDREB6 promoter was stresses induced promoter,and help to illuminate DREB′s function in improving plants resistance.
作者 李凤艳 徐兆师 李雅轩 晏月明 李连城 陈明 马有志
机构地区 首都师范大学生命科学院 国家农作物基因资源与基因改良重大科学工程
出处 《麦类作物学报》 CAS CSCD 北大核心 2011年第5期793-798,共6页 Journal of Triticeae Crops
基金 转基因生物新品种培育重大专项(2009ZX08009-083B、2009ZX08002-008B) 国家自然科学基金项目(31171546)
关键词 小麦 DREB启动子 反向PCR 瞬时表达 Wheat DREB promoter Inverse PCR Transient expression
分类号 S512.1 [农业科学—作物学] S330 [农业科学—作物遗传育种]
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参考文献20

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共引文献39

同被引文献28

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二级引证文献20

麦类作物学报
2011年 第5期

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