利用分子标记定位普通菜豆抗炭疽病基因

Mapping of Gene Conferring Resistance to Anthracnose in Common Bean(Phaseolus vulgaris L.) by Molecular Markers

为了定位中国普通菜豆的抗炭疽病基因,选取抗炭疽病地方品种红芸豆(国家库编号F2322)与高感菜豆品种京豆(国家库编号F0777)配制杂交组合,构建F2抗感分离群体和F2:3家系,用菜豆炭疽菌81号生理小种鉴定抗病性并分析遗传性。结果表明,红芸豆对菜豆炭疽菌81号小种的抗性是由一显性单基因控制的,暂将该基因命名为Co-F2322。用分离群体分组分析法(BSA)和SSR、CAPs分子标记技术,将该基因定位在B1连锁群上,利用软件Mapmaker 3.0和Mapchart 3.0计算标记与目的基因间的遗传距离,检测到3个SSR标记BMc32、C871、Pvm98和2个CAPs标记g1224、g683与抗炭疽病基因连锁,遗传距离分别为26.06、3.58、13.56、3.81和12.75cM。

To locate gene conferring resistance to anthracnose caused by pathogenic fungus（Colletotrichum lindemuthianum） from Chinese common bean,we established F2 plants and F3 families derived from a cross between red common bean cultivar F2322（resistant to race 81） and Jing common bean cultivar F0777（susceptible to race 81）,identified resistance,and did genetic analysis.The results showed that red bean carried a single dominant gene for resistance to anthracnose,designated as Co-F2322 tentatively.Molecular genetic linkage map was constructed with Mapmaker 3.0 and Mapchart 3.0.Three SSR markers（BMc32,C871,Pvm98） and two CAPs markers（g1224 and g683） that linked to the resistance gene were mapped on B1 linkage group of common bean with the distance of 26.06,3.58,13.56,3.81,and 12.75 cM,respectively.