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表皮生长因子受体单抗MAb225对舌癌细胞DNA放射后损伤修复的影响 被引量：1

Influence of epidermal growth factor receptor monoclony antibody MAb225 on DNA repair of tongue carci-noma cell after radiation
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摘要 目的研究表皮生长因子受体(EGFR)单克隆抗体MAb225对放射所致舌癌细胞DNA损伤修复的影响及其作用机制。方法应用单细胞凝胶电泳的方法检测MAb225对舌鳞癌细胞Tca8113放射后DNA双链断裂所致的尾矩,半定量逆转录多聚酶链反应和Western blot检测MAb225对DNA蛋白激酶Ku70、Ku80表达的影响。结果 MAb225作用后的细胞单细胞凝胶电泳的彗星尾矩明显高于未处理细胞(P<0.05),并能显著抑制DNA蛋白激酶Ku70、Ku80的表达。结论 MAb225通过抑制DNA蛋白激酶Ku70、Ku80的表达影响了DNA双链断裂的修复能力。 Objective The objective of this study is to investigate the effect of epidermal growth factor receptor （EGFR） monoclonal antibody MAb225 on repair of DNA double strand break （DNA-DSB） after radiation in tongue squamous cell carcinoma cell. Methods The single cell gel electrophoresis （SCCE） was performed to estimate the re- pair of DNA-DSB induced by radiation in human tongue carcinoma cells Tca8113 treated with or without MAb225. Expression of Ku70 and Ku80 were detected by semiquantitative reverse transcription polymerase chain reaction and Western bolt. Results＇ Comet tail moment of MAb225 treated cell was significantly higher than untreated cell（P〈0.05）. The expression of Ku70 and Ku80 were inhibited by MAb225. Conclusion MAb225 can inhibit repair of DNA-DSB induced by down-regulated expression of Ku70 and Ku80.
作者 王明国 王中和
机构地区 山东大学附属济南市中心医院口腔科 上海交通大学附属第九人民医院口腔颌面外科
出处 《华西口腔医学杂志》 CAS CSCD 北大核心 2011年第6期580-583,共4页 West China Journal of Stomatology
基金 山东省中青年科学家奖励基金资助项目(2006BS03046) 山东省十一五卫生科技发展计划基金资助项目(2007-11)
关键词 放射 舌癌 DNA 双链断裂 修复 radiation tongue carcinoma DNA double strand break repair
分类号 R739.86 [医药卫生—肿瘤]
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参考文献9

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二级参考文献12

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共引文献7

同被引文献14

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华西口腔医学杂志
2011年 第6期

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