白细胞介素-1β与地塞米松对成纤维细胞分泌角质细胞生长因子的影响

The effect of interleukin-1β and Dexamethasone on keratinocyte growth factor expression level of cultured oral fibroblasts

目的检测在体外培养的人正常口腔黏膜及口腔扁平苔藓(OLP)黏膜成纤维细胞中加入不同浓度的人白细胞介素-1β(IL-1β)和地塞米松(DEX)后,对成纤维细胞分泌和表达角质细胞生长因子(KGF)的影响。方法将IL-1β和DEX配制成3个浓度梯度,分别加入到体外培养的人正常口腔黏膜和OLP黏膜成纤维细胞中,收集培养72 h后各组细胞上清液,应用酶联免疫吸附剂测定(ELISA)技术检测每组细胞上清液中KGF蛋白浓度的变化。提取各组细胞RNA,用聚合酶链反应(PCR)法检测KGF mRNA表达量的变化。结果 ELISA及PCR结果显示:加入IL-1β的正常口腔黏膜及OLP黏膜成纤维细胞培养上清液中,KGF蛋白浓度较自身对照组均升高,同时KGF mRNA表达量较对照组有较明显的上升趋势。正常口腔黏膜及OLP黏膜成纤维细胞加DEX的上清液中,KGF蛋白浓度较自身对照组均降低,KGF mRNA表达量较对照组则有降低的趋势。结论 IL-1β对正常口腔黏膜成纤维细胞及OLP黏膜成纤维细胞分泌及表达KGF有促进作用;而DEX对正常口腔黏膜成纤维细胞及OLP黏膜成纤维细胞分泌和表达KGF有抑制作用。

Objective To detect the effects of human interleukin-1β（IL-1β） and Dexamethasone （DEX） on the expression level Of keratinocyte growth factor （KGF） in cultured human fibroblasts of normal oral and oral lichen planus（OLP） mucosa. Methods Three concefi[ratibn gradients of IL-1β and DEX were added to cultured fibroblasts of human normal oral and OLP mucosa respectiyely. 72 hours later, the supernatant was harvested for the detection of KGF concentration with enzyme linked immunosorbent assay （ELISA）. Total RNA of the fibroblasts was extracted and reverse transcribed. The resulting cDNA was then amplified by polymerase chain reaction （PCR） to detect the KGF mRNA. Results The results of the ELISA and PCR showed that the expression levels of KGF protein and mRNA were higher if the cells were treated with IL-1β ,However, the expression levels of KGF protein and mRNA were sig- nificantly reduced if the fibroblasts were,tread with DEX. Conclusion IL-1β can promote KGF expression levels of cultured normal oral and OLP fibroblasts, arid it is concentration-dependent. While DEX can inhibit KGF expression of cultured normal oral and OLP fibroblasts, and it is also concentration-dependent.