抗稻瘟病水稻品种Dacca6与部分杂交水稻亲本遗传多样性分析

Genetic Divergence between Blast Resistant Rice Variety Dacca6 and Some Hybrid Rice Parents

为筛选新的抗稻瘟病种质资源,选取水稻染色体上68对SSR引物,对Dacca6抗稻瘟病材料与其它27个杂交水稻之间的遗传差异进行分析。结果表明,68对引物在28份供试材料中共扩增出251条条带,63对引物具有多态性,多态性位点百分率为92.6%。每对引物扩增出条带的变幅为2～9,平均每对引物扩增出条带数为3.9。在遗传相似系数0.44处可将28份材料聚类为2类,Dacca6与这些材料的遗传差异较大,单独归为一亚类。Dacca6与各材料之间的遗传相似系数在0.330～0.517之间,平均相似系数为0.402。因此,Dacca6可被用来与其它遗传差异较大的亲本或抗源杂交,进行抗病新品种的选育。

In order to select new rice blast resistant germplasms, the genetic diversity between Dacca6 and 27 hybrid rice parents were evaluated by means of 68 microsatellites distributed over the whole rice genome. A total of 251 alleles were detected, and the number of alleles per markers ranged from 2~9, with an average of 3.9. DICE genetic similarity coefficients were calculated using the software of NTSYS-pc 2.10e and a dendrogram was constructed by the unweighted pair group method of arithmetic average （UPGMA）. All cuhivars could be divided into two groups at genetic similarities 0.44. Dacca6 with high genetic diversities could be grouped alone. The number of its genetic similarities compared with other cultivars ranged from 0.330~0.517, with an average of 0.402. The results suggested that Dacca6 with high diversities could be crossed with other cuhivars to improve rice qualities.