摘要
用土壤农杆菌C58(AgrobacteriumtumefaciensC58)对东北红豆杉(Taxuscuspidata)不同外植体和不同生长时期的愈伤组织进行了不同时间的感染,结果表明,20d龄的愈伤组织对C58菌比较敏感.感染时间为5min时冠瘿诱导率较高(15.0%).对在无激素B5培养基上得到的白色松软的冠瘿组织分析检测发现,有特异的胭脂碱(nopaline)存在,并具有产生紫杉醇的能力.
This paper demonstrates that callus of Taxus cuspidata could be transformed genetically by Agrobacterium tumefaciens C 58 and the bacterial genes transformed into the plant genome could express. Explants and calli of T.cuspidata were inoculated with C 58 for different time and the highest crown gall formation frequency (15.0%) was achieved with 20 day old callus inoculated for 5 minutes. Explants were less susceptibility than calli. In contrast to untransformed callus tissue, the crown gall could proliferate on hormone free medium and produced nopaline. By HPLC analysis, gall cells were able to synthesize taxol (0.0013% dry weight.)
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第4期27-30,共4页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
天津市自然科学基金!( 9736 0 72 1 1)
关键词
土壤农杆菌C58
东北红豆杉
紫杉醇
遗传转化
Agrobacterium tumefaciens C 58
Taxus cuspidata
callus tissue
crown gall
taxol
