C-反应蛋白在大鼠自体静脉移植动脉化中的作用

Effect of C-reactive protein on autologous vein graft arterial of rat

目的探讨C-反应蛋白(CRP)在自体静脉移植动脉化中是否参与血管重塑过程。方法通过建立自体静脉移植动脉化模型,CBS3830加以干预,应用ELISA法检测CRP水平;术后1周和2周检测内膜厚度;Western blot法检测p38、P-p38表达。结果 0～2周各时间点内,模型组CRP水平呈逐渐上升趋势,假手术组呈先上升后下降趋势,两组比较差异有统计学意义(P<0.05),在1、2周时,药物组与模型组、假手术组比较差异均有统计学意义(P<0.05)。药物组内膜增生与模型组比较差异有统计学意义(P<0.01)。1周后p38、P-p38产物表达药物组明显低于模型组及假手术组(P<0.01,P<0.05)。结论 CRP参与了自体静脉移植动脉化血管重塑过程;但可能与p38MAPK通路无关,CBS3830对其没有起到明显的抑制作用。

Objective To discuss if C-reactive protein （CRP） involved in vascular remodeling in the process of venous vein graft arterialization. Methods Interfection with CBS3830, enzyme-linked immunosorbent assay （EL- SIA） was used to detect the serum levels of CRP. Detected the intima thickness at 1,2 week in each group. Western blot was used to detect the expression of p38, p-p38. Results At 0 - 2 weeks each time point, CRP levels in the model group showed a gradual upward trend, while that in the sham group increased at first, and then decreased, with the significant difference between two groups （P 〈 0.05 ）. Compared with the sham group and model group, the drug group had significant differences at 1 week and 2 weeks time （ P 〈 0.05 ）. Intimal hyperplasia of drug group,compared with the model group has differences （P 〈0.01 ）. 1 week later shows the product of p38 and P-p38 expression, drug group was significantly lower than the other two groups （ P 〈 0.01 ）. Conclusion CRP take part in the autologous vein graft arterial vascular remodeling process; which may not be related with the p38MAPK pathway for CBS3830 does not play a significant inhibiting effect.