三七皂苷单体R_1对低氧高二氧化碳肺动脉平滑肌细胞ERK1/2表达的影响

Effects of Notoginsenoside monomer R_1 on the expression of ERK1/2 in PASMCs

目的:探讨三七皂苷单体R1减轻低氧高二氧化碳性肺动脉收缩(hypoxia hypercapnia-in-duced pulmonary vasoconstriction,HHPV)的作用及其与细胞外信号调节激酶(ERK)1/2信号通路的关系。方法:原代培养雄性SD大鼠肺动脉平滑肌细胞(PASMCs),随机分为6组:常氧组(N组),低氧高二氧化碳组(H组),DMSO对照组(HD组),R1干预组(R8、R40、R100组)。采用免疫印迹法测定ERK1/2磷酸化蛋白表达,半定量逆转录-聚合酶链反应技术检测ERK1、ERK2基因表达水平。结果:p-ERK蛋白在N组表达弱,与H、HD组比较,R8、R40、R100组均不同程度下调,以R8组为著,差异有统计学意义(P<0.01);ERK1mRNA、ERK2mRNA在N组弱表达,与H、HD组比较,R8、R40、R100组表达均不同程度降低(P<0.01和P<0.05),以R8组为著。结论:ERK1/2信号通路可能介导大鼠低氧高二氧化碳性肺动脉收缩;三七皂苷单体R1可能通过抑制ERK1/2通路减轻低氧高二氧化碳性肺动脉收缩。

AIM：To study the effect of monomer R1 aleviating HHPV via MAPKs signal pathway.METHODS： To culture pulmonary arterial smooth muscle cells（PASMCs） of male SD rats primarily,PASMCs were randomly divided into 6 groups： control group（N）,hypoxia hypercapnia group（H）,DMSO incubation group（HD）,R1 groups（R8,R40,R100）.Incubated cells were collected.p-Erk1/2 was investigated through western blot,and ERK1 mRNA and ERK2 mRNA were detected via RT-PCR.RESULTS：The expretion of p-ERK protein was weak in the N group,and in R8,R40 and R100 groups the level of p-ERK was reduced significantly（P0.01）,declining mostly in R8 group.The expretion of ERK1 mRNA and ERK2 mRNA was not obvious in N group,and comparing with H and HD groups it markedly down-regulated in R8,R40 and R100 groups（P0.01,P0.05）,reaching its lowest point in R8 group.CONCLUSION： ERK1/2 signal pathway may play an important role in HHPV in rat.The effect of R1 on relaxing HHPV may be related to inhibiting expression of ERK1/2.