血浆循环microRNA-126在非小细胞肺癌诊断中的应用

Application of plasma circulation microRNA-126 to diagnosis of non-small cell lung cancer

目的探讨血浆循环microRNA-126浓度与非小细胞肺癌(NSCLC)的相关性及其诊断价值。方法采用实时荧光定量PCR方法检测130例NSCLC患者和作为对照的170例健康人的血浆循环microRNA-126浓度。结果对照组血浆循环microRNA-126浓度显著高于NSCLC组(P<0.01)。血浆循环microRNA-126浓度ROC曲线下面积(area under theROC curve,AUC)为0.758,95%CI为0.704～0.811(P<0.01)。当血浆microRNA-126浓度为1051 fmol/L时,血浆microRNA-126对NSCLC诊断的特异度为90.0%,灵敏度为46.4%。其浓度与患者的年龄、性别、吸烟,病理分型和分期均无显著的相关性(P>0.05)。结论血浆循环microRNA-126浓度对NSCLC有一定的临床诊断意义,联合其他血浆microRNA或肿瘤标志物,如神经元特异性烯醇化酶、癌胚抗原和细胞角蛋白片段21-1,可以弥补单一检测的局限性,以提高肺癌的阳性检出率,为临床提供可靠的依据。

Objective To discuss the correlation between the concentration of plasma circulation microRNA-126 and the non-small cell lung cancer（NSCLC）,and to study its diagnostic value.Methods The concentration of plasma circulation micro RNA-126 in 130 patients with NSCLC（NSCLC group）and 170 healthy people（control group）was detected through the method of real time fluorescent quantitation PCR.Results The concentration of microRNA-126 in the control group was significantly higher than that in the NSCLC group（P0.01）.The area under the receiver operating characteristic curve of the concentration was 0.758,and the 95% CI was 0.704-0.811（P0.01）.When the concentration of plasma microRNA-126 was 1051 fmol/L,its specificity in the diagnosis of NSCLC was 90%,and its sensitivity was 46.4%.There were not obvious correlations between the concentration and age,gender,smoking,pathological typing and staging of the 130 patients.Conclusion The concentration of plasma circulation microRNA-126 has certain significance in the diagnosis of NSCLC.Combined with other plasma microRNA or tumor markers,such as neuron-specific enolase,carcino-embryonic antigen and cytokeratin fragment 21-1,it can retrieve the limitations of the simple detection,increase the positive detection rate of lung cancer and provide dependable data for clinical diagnosis.