高效液相色谱法测定五参安神胶囊中丹参酮ⅡA的含量

Determination of content of tanshinone ⅡA in Wushen Anshen capsule by high performance liquid chromatography

目的建立五参安神胶囊中丹参酮ⅡA含量的测定方法。方法甲醇超声处理样品及对照品后,采用高效液相色谱法,Agilent Zorbax B-C18柱,以甲醇-水(85∶15)为流动相,检测波长270 nm,流速为1.0 ml/min对其进行测定。结果丹参酮ⅡA在浓度为5～120μg/ml范围内与峰面积呈良好的线性关系,r=0.9999,平均加样回收率为100.76%,RSD0.33%。结论该方法简便,结果准确,可用于五参安神胶囊的质量控制。

Objective To establish a method to determine the content of tanshinone ⅡA in Wushen Anshen capsule.Methods The samples and the control articles were ultrasonicated by methanol,and then detected by high performance liquid chromatography（HPLC）.In the detection,the Agilent Zorbax B-C18（4.6 mm ×250 mm,5 μm）was used.The mobile phase was methanol to water（85∶ 15）.The detection wavelength was 270 nm and the flow rate was 1.0 ml/min.Results Confined in the content of 5-120 μg/ml（r=0.9999）,tanshinone ⅡA had a fine linear relationship with the peak area.The average recovery rate with added samples was 100.76%,and the RSD was 0.33%.Conclusion Determination of tanshinone ⅡA content by HPLC is simple and accurate.It can be used for quality control of Wushen Anshen capsule.