单价流感疫苗脂质体干粉细胞免疫研究

Cellular immunity of monovalent influenza vaccine lyophilized liposome

从细胞免疫水平考察流感疫苗脂质体干粉肺部免疫的免疫原性,以验证在稳定性提高的同时,流感疫苗脂质体干粉肺部免疫原性不低于现行应用的流感疫苗原液腹腔注射免疫。将实验小鼠分为2个大组,每组分为阴性对照组、疫苗脂质体冻干粉组、非脂质体流感疫苗原液组和阳性对照组(n=5)。非脂质体流感疫苗原液组和疫苗脂质体冻干粉组分别以每只6μg血凝素(以H1N1计)肺部灌注免疫,同时以每只6μg非脂质体流感疫苗原液组腹腔免疫作为阳性对照。分别免疫14 d和28 d后,用四甲基偶氮唑盐微量酶反应比色法(MTT法)检测脾淋巴细胞增殖情况,以考察其细胞免疫原性。脂质体肺部免疫可以诱导细胞免疫,且其免疫原性明显高于流感疫苗原液传统腹腔注射免疫组。与流感疫苗原液腹腔注射免疫相比,流感疫苗脂质体干粉通过肺部免疫,细胞免疫效果明显提高。

To evaluate the immunogenicity of the influenza vaccine lyophilized liposomes through cellular immunity,it could be confirm that,with the increasing of the stability,the immunogenicity of the influenza vaccine lyophilized liposomes by pulmonary delivery was better than that of the influenza vaccine non-liposome immunized by intraperitoneal injection.Experimental mice were divided into two groups,and each group was divided into the negative control group,the influenza vaccine lyophilized liposome group,the influenza vaccine non-liposome group,and the positive control group（n=5）.The influenza vaccine lyophilized liposome group and the influenza vaccine non-liposome group were immunized with 6 μg hemagglutinin of H1N1 per mouse through pulmonary delivery,and the positive control group was immunized with 6 μg hemagglutinin of H1N1 per mouse through intraperitoneal injection.MTT method was used to measure the spleen cell proliferation after immunization to mice 14 days and 28 days in order to study the immunogenicity of the influenza vaccine lyophilized liposome.The influenza vaccine lyophilized liposome could induce the cellular immunity of mice by pulmonary delivery and the level of the cellular immunity was higher than that of the influenza vaccine non-liposome immunized through intraperitoneal injection.Pulmonary delivery of the influenza vaccine lyophilized liposome could achieve satisfied cellular immunity effect.