急性髓系白血病与急性淋巴系白血病的蛋白质组比较研究

Comparison of Proteomics between Acute Myeloid Leukemia and Acute Lymphoid Leukemia

本研究通过对急性髓系白血病(AML)与急性淋巴系白血病(ALL)细胞比较蛋白质组分析,寻找亚型特异性的蛋白质表达谱。应用双向电泳分别分离AML不同亚型(M1、M2、M3)、ALL患者骨髓白血病细胞蛋白质,利用PDQuest 7.4软件分析双向电泳图谱差异蛋白质点,基质辅助的激光解析飞行时间质谱和生物信息学鉴定差异蛋白质。结果显示:经过电泳图谱分析得到21个差异蛋白质点,质谱鉴定提示15种蛋白有统计学意义。在AML中高表达的蛋白质包括髓过氧化物酶(MPO)、硫氧还蛋白依赖的过氧化物还原酶(PRDX3),钙网蛋白(CALR)、烯酰辅酶A水合酶ECH1等7种,在ALL中高表达的蛋白质包括ARHGDIB、肌动蛋白抑制蛋白1(PFN1)、肌动蛋白(ACTG1)等8种。结论:AML与ALL细胞存在差异蛋白质表达谱,这将有助于发现早期诊断的分子标志物及特异性治疗靶标。

The aim of this study was to explore the distinct protein profiles of different subtype of acute myeloid leukemia（AML）,including M1,M2,M3 and acute lymphoid leukemia（ALL） by differential proteomic expression analysis.The proteins of bone marrow leukemia cells from AML and ALL patients were separated by two-dimensional electrophoresis（2-DE）.2-DE patterns were analyzed by PDQuest 7.4 software and the differentially expressed proteins were identified by matrix-assisted laser desorption ionization-time of flight mass spectromety（MALDI-TOF-MS） and bioinformatics.The results indicated that 21 differentially expressed proteins were found by 2-DE and 15 were identified by MS to be significantly differentially expressed.In AML,seven proteins were highly expressed such as MPO,PRDX3,CALR and ECH1 and so on,and eight proteins were highly expressed in ALL,including ARHGDIB,PFN1 and ACTG1 and so on.It is concluded that the distinct protein profiles between AML and ALL have been proved.It may be helpful for the identification of new targets for specific treatment approaches and the molecular markers for the early diagnosis of leukemia.