109例慢性B淋巴系白血病免疫表型特征分析

Analysis of Immunophenotype Characteristics in 109 Cases of B lineage Chronic B Lymphoid Leukemia

本研究目的在于了解慢性B淋巴系白血病(B-CLL)的免疫表型特征,为临床的诊断、治疗、微小残留病灶检测及预后判断提供依据。应用流式细胞术及一组单克隆抗体,通过双色/三色流式细胞术,采用细胞表面与胞浆联合标记的方法,对109例序贯而来的B-CLL病例进行免疫表型分析。结果表明,109例B-CLL均表达CD19,其他B系抗原CD20、CD22、CD23阳性率分别为95.40%、94.50%、86.20%,无1例表达CD10。在105例B-CLL中有28.60%表达FMC-7,36.20%表达CD38。在所有B-CLL中CD5+B-CLL占86.23%,CD5-B-CLL占13.76%。对50例B-CLL进行ZAP-70的检测,其中12例ZAP-70阳性。在73例B-CLL中,膜表面球蛋白κ轻链阳性多于λ轻链。结论:了解B-CLL的免疫表型特点对此类疾病的诊断及治疗后微小残留病灶的检测具有重要意义,而免疫表型与预后的关系则需作进一步研究。

This study was aimed to investigate the immunophenotypic characteristics of 109 cases of B-cell chronic lymphoid leukemia（B-CLL） so as to provide evidences for the diagnosis and therapy of B-CLL,and for the detection of the minimal residual disease and its prognosis.Immunophenotyping was performed in 109 patients of B-CLL by two/three color multiparameter flow cytometry analysis using a panel of monoclonal antibodies.The results showed that in 109 cases of B-CLL,all cases expressed CD19,the positive ratios of other B lineage antigen such as CD20,CD22 and CD23 were 95.40%,94.50%,86.20% respectively.None of the B-CLL cases expressed CD10.The expression ratio of FMC-7 and CD38 in 105 cases of B-CLL were 28.60 % and 36.20%.Among the B-CLL cases the CD5^＋ cells amounted to 86.23%,CD5^-cells amounted to 13.76%,ZAP-70 protein was expressed in 12 out of 50 patients.It is concluded that immunophenotypic data are very useful for the diagnosis and detection of minimal residual disease of B-CLL,and the relationship between the immunophenotypic characteristics and the prognosis of B-CLL needs further study.