摘要
本研究探讨骨髓增生异常综合征(MDS)中骨髓基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)的表达,及其与骨髓CD34+细胞的凋亡和骨髓血管新生的关系。搜集40例MDS病例,根据IPSS积分分为低危和高危2组,采集患者的骨髓穿刺物和活检标本。检测骨髓血浆中SDF-1的含量,骨髓CD34+细胞的凋亡率,骨髓活检标本的微血管密度(MVD)。结果显示:SDF-1α在低危组和高危组的表达量分别为(2313±417)pg/ml,(1241±501)pg/ml,前者显著高于后者(p<0.05);且2组的表达率均显著高于健康正常者(710±153)pg/ml(p<0.05);Annexin-Ⅴ/PI双染色显示,低危MDS患者CD34+细胞的凋亡率为(54.75±14.15)%,显著高于对照组的(18.51±8.66)%和高危组的(23.96±12.20)%(p<0.05),后两者相比差异无显著性(p>0.05);相关分析显示:低危组中骨髓SDF-1含量与CD34+细胞凋亡率呈正相关(p<0.05);在高危组中骨髓SDF-1含量与MVD呈现正相关(p<0.05)。结论:骨髓SDF-1含量、CD34+细胞凋亡和MVD在MDS中存在显著异常,在不同风险度的病例中也有明显的不同,且SDF-1水平与骨髓细胞的凋亡和血管新生具有相关性。
The study was aimed to investigate the expression of stromal cell derived factor（SDF-1） in bone marrow（BM） and its relation with apoptosis of BM CD34^＋ cell and angiogenesis in myelodysplastic syndrome（MDS）.40 patients with MDS were divided into low-risk group and high-risk group according to IPSS score system.BM samples were collected.SDF-1 levels,the apoptosis of CD34^＋ cells and microvessel density（MVD） of BM were detected by ELISA,flow cytometry and immunochemistry,respectively.The results showed that the SDF-1 level in MDS patients was significantly higher than that in normal controls（p〈0.05）,and SDF-1 level in low-risk group was significantly higher than that in high-risk group.Apoptosis of CD34^＋ cells significantly increased in low-risk group compared with other groups（p〈0.05）.MVD in BM biopsy significantly increased in high-risk MDS group（p〈0.05）,compared with low-risk MDS group which also had higher MVD than the control group（p〈0.05）.Positive correlation was found between apoptosis of CD34^＋ cells and SDF-1 levels in low-risk group,and SDF-1 level and MVD in high-risk group.It is concluded that the expression of SDF-1,apoptosis of BM CD34^＋ cells and MVD were significangtly abnormal in MDS patients,especially in different risk group,suggesting that SDF-1 level is related to cell apoptosis and angiogenesis.
出处
《中国实验血液学杂志》
CAS
CSCD
2011年第6期1438-1442,共5页
Journal of Experimental Hematology
基金
国家自然基金面上项目(编号30672208)
