犬细小病毒病的PCR诊断

Diagnosis for canine parvovirus disease by PCR assay

根据GenBank中收录的犬细小病毒(CPV)AY787930.1株设计一对特异性引物,建立了一种快速、准确的诊断CPV的PCR方法.从12份疑似犬细小病毒病的粪便中提取DNA,并以此DNA为模板,进行PCR扩增和克隆及测序.应用该方法对12份临床病料进行PCR扩增,有11份为阳性,1份为阴性;对11份阳性片段的克隆、测序结果与AY787930.1株的相应片段相比,同源性为99.9%.该方法适用于所有易感动物的犬细小病毒的临床诊断,与胶体金试纸相比具有快速、准确和特异性好的优点.

Based on the published sequence of canine parvovirus（CPV） strain AY787930.1 in GenBank,a pair of specific primers are designed,and a rapid and accurate PCR method is established for detecting CPV.The total DNA is extracted from 12 feces samples of virus-infected dog showing enteritis symptoms,and taking the total DNA as a template,the PCR amplification,cloning and sequencing of the target fragment is conducted.The results show that among 12 clinical samples detected by PCR,11 are positive and 1 is negative,and the homology of 11 positive fragments is 99.9% with the strain of AY787930.1.Compared with gold immunochromatographic assay,this method has advantages of rapidity and accuracy.The PCR assay in this paper is a good method for all sensitive animals diagnosis of CPV disease.