高分子聚合物微流控细胞芯片的改性研究

Surface modification of polymer microfluidic cell chip

目的探讨2种不同硅烷化试剂对高分子聚合物材料PDMS芯片的改性效果和改性后芯片在细胞捕获/筛选的初步应用。方法接触角实验考察不同硅烷化试剂处理的PDMS样本亲水性,筛选硅烷化试剂及其浓度,以芯片上的非特异性细胞捕获实验验证筛选结果,并连接抗体构建功能化芯片,进行细胞捕获实验的初步研究。结果 2种硅烷化试剂均对PDMS表面具有改性作用,其中在体积浓度为2%GPTMS处理后的芯片表面测得的接触角值最小,芯片对细胞非特异性吸附为5.3%,固载抗体后芯片对细胞的净捕获率为53.96%。结论本研究筛选出PDMS硅烷化反的试剂为GPTMS,最佳浓度为2%,此条件下材料的亲水性良好,对细胞的非特异性吸附低,可用于功能化细胞捕获芯片的构建。

Objective To determine the effect of two different silylation reagents on surface modification of polydimethylsiloxane（PDMS） polymer microfluidic cell chip and investigate the cell capture/screen initial application of the modified microfluidic chip.Methods Hydrophilicity of samples treated by silylation reagents was measured by contact angle to screen silane reagent and determine optimal concentration.The results were verified by non-specific cell capture experiments.Then immobilized antibody was used to establish functional cell chip for further study on cell capture.Results In this study,both two silylation reagents reduced the non-specific adsorption.GPTMS at 2%（volume concentration） obtained a smallest contact angle and resulted in non-specific adsorption of cells reduced to 5.3%.The capture rate was increased to 53.96% after antibody immobilization.Conclusion An effective silylation reagent GPTMS is screened and its best concentration for surface modification of PDMS chip is 2%.In this condition,PDMS is hydrophilic and has a lower adsorption for non-specific cells.This modification is benefit to preparation of functioned cell capture chips.